Proliferative response of IGF-II and rhIns through IR-B. A. HeLa cells transiently transfected with pcDNA3-IR-B or EV were assayed by Western blot detecting the expression of IR and ERK 1/2 (loading control). B. MTS assay in cells transiently transfected with pcDNA3-IR-B or EV stimulated with 0.1 nM, 1.0 nM and 10.0 nM IGF-II or rhIns for 2 days. The results are shown as the mean ± s.e.m (n = 3 independent experiments). Asterisks indicate significant differences between IR and EV (p < 0.05).