Subcellular localization of TSPO-eGFP in RBL-2H3 cells. RBL-2H3 cells were transiently transfected with TSPO-eGFP and stained with MitoTracker Red CMXRos. TSPO-eGFP (A) and MitoTracker (B) fluorescence were detected approximately 48 h after transfection. Resulting signals were analyzed and merged (D) with Zeiss ZEN 2009 software. Brightfield images (C) of the cells were added for reference. White bars (upper right corner) equal 20 μm. Similar results were obtained for at least 10 different cells from 3 independent experiments. (E) Image magnification of (D) showing one of the central cells to enable better visual analysis of the plasmalemnal area.