Suppression of Ag-, LPS-, and SCF-triggered IL-6 production by Ro5-4864. IgE-loaded BMMCs were pretreated with DMSO, Ro5-4864 or clonazepam for 20 min and subsequently stimulated with 20 ng/ml Ag (A), 5 μg/ml LPS (B) or 150 ng/ml SCF (C) for 3 h or left unstimulated. Subsequently, IL-6 concentrations in the supernatants were determined by ELISA. Each bar is the mean of triplicates ± SEM. Comparable results were obtained with cells from different cultures (n≥3). Marks of significance (“asterisks”) relate to the respective vehicle (DMSO) control.