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Figure 4 | Cell Communication and Signaling

Figure 4

From: Cytosolic Ca2+ shifts as early markers of cytotoxicity

Figure 4

Assessment of As 2 O 3 , gossypol, H 2 O 2 and staurosporine-induced toxicity in MCF-7 cells. (A) Upper panel: Alamar Blue assay in presence of As2O3 as indicated (mean±SD; n≥4). Lower panel: Fluo-4 analysis of 20 μM and 50 μM As2O3 treated cells (mean±SD; *p<0.05; n=3; t test) (B) Upper panel: Alamar Blue assay in presence of gossypol as indicated (mean±SD; n≥4; n.s. not significant; t test). Lower panel: Fluo-4 analysis of 60 μm and 75 μM gossypol treated cells (mean±SD; *p<0.025; n=4; t test) (C) Upper panel: Alamar Blue assay in presence of H2O2 as indicated (mean±SD; n≥4). Lower panel: Fluo-4 analysis of 5 mM and 10 mM H2O2 treated cells (mean±SD; *p<0.005; n≥3; t test) (D) Upper panel: Alamar Blue assay in presence of staurosporine as indicated (mean±SD; n≥4). Lower panel: Fluo-4 analysis of 200 nM and 400 nM staurosporine treated cells (mean±SD; *p<0.05; n≥3; t test).

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