Figure 3
From: Cytosolic Ca2+ shifts as early markers of cytotoxicity
Assessment of As 2 O 3 , gossypol, H 2 O 2 and staurosporine-induced toxicity in HeLa cells. (A) Upper panel: Alamar Blue assay in presence of As2O3 as indicated (mean±SD; n≥3; n.s. not significant; t test). Lower panel: Fluo-4 analysis of 5 μM and 50 μM As2O3 treated cells (mean±SD; *p<0.025; n=3; t test) (B) Upper panel: Alamar Blue assay in presence of gossypol as indicated (mean±SD; n≥3). Lower panel: Fluo-4 analysis of 75 μm and 100 μM gossypol treated cells (mean±SD; *p<0.0025; n≥4; t test) (C) Upper panel: Alamar Blue assay in presence of H2O2 as indicated (mean±SD; n≥4). Lower panel: Fluo-4 analysis of 0.5 mM and 2 mM H2O2 treated cells (mean±SD; *p<0.025; n≥3; t test) (D) Upper panel: Alamar Blue assay in presence of staurosporine as indicated (mean±SD; n≥3). Lower panel: Fluo-4 analysis of 400 nM and 1000 nM staurosporine treated cells (mean±SD; *p<0.05; n=3; t test).