Figure 9
From: Invasion of epithelial cells by Campylobacter jejuni is independent of caveolae
Treatment of HeLa cells with methyl-β-cyclodextrin (MβCD) disrupts the C. jejuni -dependent association of phospho-EGF receptor (pEGFR) with β 1 integrin. HeLa cells were infected with C. jejuni in the presence and absence of 5 mM MβCD for 45 min. Panels: A) Cell lysates were immunoprecipitated with an EGFR antibody, separated by SDS-PAGE, and blotted for β1 integrin, pEGFR, and total EGFR (loading control). HeLa cells were uninfected (Lanes 1 and 3) or C. jejuni infected (Lanes 2 and 4) in the absence (Lanes 1 and 2) or presence of MβCD (Lanes 3 and 4). Also shown are the blots of the IgG isotype control IP probed with an antibodies reactive against the EGFR and the β1 integrin. B) Quantification of band intensity of β1 integrin and pEGFR from three independent experiments. The asterisk indicates P < 0.01 by one-way ANOVA followed by post-hoc Dunnet’s analysis.