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Figure 8 | Cell Communication and Signaling

Figure 8

From: Invasion of epithelial cells by Campylobacter jejuni is independent of caveolae

Figure 8

Knockdown of endogenous caveolin-1 protein does not alter C. jejuni -induced membrane ruffling as judged by scanning electron microscopy (SEM). HeLa cells were transfected with caveolin-1 siRNA or scrambled (Scr) siRNA, infected with C. jejuni for 15 min, and processed for examination by SEM as outlined in ‘Methods.’ The images shown are uninfected HeLa cells (Panels A and F) and C. jejuni infected HeLa cells (Panels B-E and G-J) that are untreated (Panels B and G), treated with caveolin-1 siRNA (Panels C and H), scrambled (Scr) siRNA (Panels D and I), or with the FAK inhibitor TAE 226 (Panels E and J). Arrows indicate C. jejuni associated with the host cells. Also indicated is the percentage of cells with membrane ruffling of total cells (N). In contrast to the C. jejuni infected cells in the absence of an inhibitor, C. jejuni infected cells treated with caveolin-1 siRNA, and C. jejuni infected cells treated with Scr siRNA, treatment of cells with the FAK inhibitor TAE 226 inhibited C. jejuni-induced membrane ruffling. The images shown in Panels A-E were collected at the same magnification and the images shown in Panels F-J were collected at the same magnification.

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