Figure 5
From: Invasion of epithelial cells by Campylobacter jejuni is independent of caveolae
C. jejuni induces the association of β 1 integrin and EGF receptor (EGFR) independent of caveolin-1. HeLa cells were treated with caveolin-1 (Cav-1) siRNA and scrambled (Scr) siRNA. The cells were infected with C. jejuni and incubated for 30 min. A portion of the whole cell lysate (WCL) was probed for actin (loading control) and caveolin-1 to determine the efficacy of the siRNA treatment (top two blots). The EGFR was then immunoprecipitated (IP) from the WCL, separated by SDS-PAGE, and blotted for EGFR and β1 integrin. The EGFR coprecipitated with the β1 integrin following C. jejuni infection. This association was not disrupted following caveolin-1 knockdown. Also shown is a blot of the IgG isotype control IP probed with an antibody reactive against the β1 integrin.