Figure 4

Treatment of HeLa cells with MβCD disrupts C. jejuni -dependent EGF receptor (EGFR) phosphorylation and prevents EGFR association with caveolin-1. HeLa cells were infected with C. jejuni with and without 5 mM MβCD treatment for 45 min. Panels: A) Cell lysates were immunoprecipitated (IP) with an EGFR antibody, separated by SDS-PAGE, and blotted for pEGFR, total EGFR, and caveolin-1. Lanes: 1, Uninfected cells (Cells only); 2, Infected with C. jejuni in the absence of MβCD (vehicle only, water); 3, Infected with C. jejuni in the presence of 5 mM MβCD. Also shown are the blots of the IgG isotype control IP probed with EGFR and caveolin-1 antibodies. B) Quantification of band intensity of pEGF receptor from three independent experiments. The asterisk indicates P < 0.01 by Student’s t test.