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Figure 6 | Cell Communication and Signaling

Figure 6

From: Altered responsiveness to extracellular ATP enhances acetaminophen hepatotoxicity

Figure 6

Up-regulation of several purinergic receptors may explain hyper-responsiveness to ATP during hepatotoxicity. (A) Quantitative PCR for purinergic receptors from cells cultivated in the presence or absence of APAP (20 nM; 24 h). Untreated HepG2 cells (C) determined baseline expression. (B-C) HepG2 incubation with adenosine in the presence or absence of APAP. (D) Serum ALT levels of APAP-challenged mice treated with theophylline (P1 receptor blocker, 20 mg/Kg). (E) Quantitative PCR analysis of liver samples from acute hepatitis patients in comparison to healthy volunteers. Baseline expression was determined by choosing a healthy volunteer as control. (F) Changes in expression of different P2 receptors were compared to serum ALT levels from healthy and acute hepatitis patients. Note that increased P2R expression was correlated with higher serum ALT levels in several patients. Pearson’s correlation was calculated comparing fold increase of P2R expression to serum ALT levels (r). * P < 0.05 in comparison to control group and ** in comparison to vehicle treated group.

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