Figure 3
From: Altered responsiveness to extracellular ATP enhances acetaminophen hepatotoxicity
ATP is released and accounts to additional cell death during APAP incubation. (A-B) Dose and time-response curves of APAP challenged HepG2 cells (24 h). (C-E) Acridine orange/ethidium bromide viability test confirmed APAP cytotoxicity. (F) ATP and ADP quantification in HepG2 supernatant (HPLC) in different timepoints after APAP incubation (20 mM). (G-H) Effects of apyrase (10 U/ml) or different P2, P2X and P2Y blockers (0.1 mM, 100 μM and 30 μM, respectively) on APAP-mediated cell death. (I-J) Direct cytotoxic effect of ATP and ADP on “naïve” HepG2 cells, confirmed by acridine orange/ethidium bromide viability test. Controls (medium alone) were not different throughout the experiments. * P < 0.05 in comparison to control group and ** in comparison to vehicle or medium treated group.