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Figure 6 | Cell Communication and Signaling

Figure 6

From: Epstein-Barr virus LMP2A signaling in statu nascendi mimics a B cell antigen receptor-like activation signal

Figure 6

The LMP2A N-terminus modulates BCR signaling. DT40 transfectants were induced with 4-HT to express chimeric CD72/LMP2A, loaded with Indo-1 and stained with FITC-labeled anti-CD72 in order to gate on CD72 negative and CD72 positive cells (upper panels). Subsequently, BCR-induced Ca2+ responses were monitored by flow cytometry (lower panels) upon stimulation of the cells with anti-IgM antibodies (blue and red curves) or with anti-mouse IgG F(ab)2 fragments to super-crosslink the anti-CD72 antibody (purple and yellow curves). Blue and yellow lines represent CD72/LMP2A-negative cells, red and purple lines CD72/LMP2A-positive cells. (A) and (B) represent two indepent cell clones.

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