Global gene expression changes of in vitro stimulated human transformed germinal centre B cells as surrogate for oncogenic pathway activation in individual aggressive B cell lymphomas

Table 1 Differential expression in human transformed germinal centre B cells in response to B cell-specific stimulations

	α-IgM	CD40L	IL21	LPS	BAFF
Upregulated genes	3039	689	463	114	69
Downregulated genes	3557	496	439	169	39
Total number of genes affected	6596	1194	902	283	129

BL2 cells stimulated through αIgM treatment, by CD40L, IL21, BAFF or LPS. RNA was hybridized onto U133A 2.0 plus Arrays. Differentially expressed genes between stimulated and control cells were identified using linear models as implemented in the bioconductor package LIMMA [36]. False discovery rates for lists of differentially expressed genes were calculated according to Benjamini and Hochberg [38]. Genes were ranked according to their p-value for differential expression from the microarray experiments (adj. P value ≤ 0.05).

ISSN: 1478-811X