Pathways involved in the regulation of a selected set of genes in response to αIgM treatment. (A) Prior knowledge scheme of monitored pathways. Dashed lines implicate the presence of additional signalling molecules interlinking the depicted factors, solid lines reflect a direct link. (B) hierarchical presentation of kinases affected by utilized chemical inhibitors . (C-N) BL2 cell were preincubated for 3 hrs with 2 μM SB203580 (p38), 10 μM SP600125 (JNK), 10 μM U0126 (MAP2K), 100nM 5Z-7-oxozeaenol (TAK), 7 μM ACHP (IKK2) or 10 μM Ly294002 (PI3K) inhibitors and then stimulated by 1.3 μg/ml αIgM F(ab)2 fragments for additional 3 hrs in the presence of respective inhibitors. Cells were harvested to isolate RNA for corresponding qRT-PCR. Expression of the following genes is shown: SGK1, PYGO1, SLAMF3, EGR2, ID3, CCR7, DUSP2, SLAMF6, MYC, LEF1, IRF4 and RGS1. Results are presented as 2-ΔΔCT values, relative to abl housekeeper expression and compared to the corresponding unstimulated inhibitor treated control. As RGS1 (N) expression is below detectable levels in unstimulated probes, only ΔCt values relative to stimulated control without inhibitors were compared. One representative experiment out of three or more biological replicates is shown. Cells were treated with DMSO (1/8) 5Z-7-oxozeaenol (2/9), ACHP (3/10), SB203580 (4/11), SP600125 (5/12), Ly294002 (6/13), U0126 (7/14) in the absence (1–7) or presence of αIgM F(ab)2 fragments (8–14). n.e. – not expressed. Details on pathway activations as measured by immunoblot, kinase activity and Ca2+ influx analysis are summarized in Additional file 24: Figure S3.