Figure 4
Changes in global gene expression separates Burkitt lymphoma and diffuse large B cell lymphoma. The expression of the TOP100 most prominent responding genes upon stimulation of BL2 with αIgM F(ab)2 fragments was investigated in primary lymphoma. Left panel: 220 lymphoma cases [20]; right panel: 99 lymphoma cases [21]. NHL cases were ordered from left to right according to their expression of αIgM affected genes in BL2 cells. The Heatmaps display the expression of target genes (columns) across lymphoma samples (rows). The lower colour bar above the heatmaps marks mBL in red, non-mBL in green and intermediate lymphoma in yellow (molecular diagnosis). The affiliation of samples to ABC/GCB DLBCL subgroups and the presence of an IG-MYC translocation is encoded in the middle and upper bar on top of the map (see legend for colour coding). Relative gene expression is encoded with yellow (high expression) and blue (low expression). As different microarrays were used for the whole genome expression analyses of cell perturbation and patient samples (Affymetrix HGU-133A and HGU133 plus2.0), the list of TOP100 genes had to be adapted to be able to transfer the resulting genes to patient data (see also Table 3, Material and Methods section and Supplementary material for additional details).