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Figure 3 | Cell Communication and Signaling

Figure 3

From: Interferon-γ-induced activation of Signal Transducer and Activator of Transcription 1 (STAT1) up-regulates the tumor suppressing microRNA-29 family in melanoma cells

Figure 3

Inverse correlation of miR-29a expression levels and melanoma proliferation. Comparison of basal expression levels of (A) primary miRNA clusters pri-29a~b-1 (blue/red/black bars) and pri-29b-2~c (grey bars) and (B) mature miR-29a (blue/red/black bars) and miR-29b (grey bars) in NHEM-M2, eight melanoma cell lines and HaCaT keratinocytes. Graphs show 2-Δct x102 with SD of biological triplicates. (C) Mean growth curves of untreated melanoma cell lines over 4 days (biological quadruplicates). Melanoma cell lines with ‘low expression’ of pri-29a~b-1 and miR-29a show faster proliferation whereas cells with a relatively ‘high expression’ proliferate slower. (D,E) Proliferation assay of (black) mimic/inhibitor- and (grey) NC-mimic/NC-inhibitor-transfected cells over 72h in (D) A375 and (E) FM55P cells; representative graphs of four independent experiments. Error bars depict SDs of technical triplicates. The inserted graphs (upper left corners) show the mean confluence of 4 biological replicates at 0h and 72h time points of the proliferation assay. Depicted are ratios of confluence of 29ab-mimic/NC-mimic treated cells (D) and 29a-inhibitor/NC-inhibitor treated cells (E). Error bars show SEM. Significance was assessed by a two-tailed t-test with * p<0.05, ** p<0.01 and *** p<0.001.

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