Figure 6
Measurement of neutrophil numbers and Cxcl1 expression in Control and TgS100a8a9hepmice treated with doxycycline. Control (Co) and TgS100a8a9hep (Tg) mice were treated with (Co +dox, Tg +dox) or without (Co -dox, Tg -dox) doxycycline (10 μg/ml) containing drinking water. (A) Relative expression of Cxcl1 was determined by qRT-PCR on liver cDNA. Mean +SD, students t-test, *p≤0.05, **p≤0.01, n.s. not significant. (B) Peripheral blood cells were stained with specific antibodies for Cd11b (APC), Gr1 (PE) and Ly-6G (FITC) followed by flow cytometry analysis. Box-plot shows the relative fold increase of Gr1+/Ly-6G+ population from all Cd11b+ cells in peripheral blood; Median, 25% and 75% quartile (light grey boxes), students t-test, *p≤0.05. (C) IHC staining with Gr1-specific antibodies was performed on liver sections and representative images (n=4 animals per group) are shown with red staining for positively stained cells and counterstaining with hematoxilin. Bars represent 200 μm.