Figure 4
Molecular markers of neutrophil activation in TgS100a8a9hepmice. Blood neutrophils (Gr1+/Ly-6G+ or Cd11b+/Gr1+) from Control and TgS100a8a9hep mice or isolated neutrophils treated with 10 μg/ml TPA for 5 min (Pos) were stained with specific antibodies for (A) Cd11b (APC) and (B) L-Selectin (FITC), or the respective isotype control antibody. The mean fluorescence intensities from Control (Cd11b n=9; L-Selectin n=5), TgS100a8a9hep (Cd11b n=8; L-Selectin n=5) and Pos (n=3) samples were determined and depicted as means +SEM.