Figure 3
Increased amount of neutrophils in peripheral tissue and blood samples. (A) Relative expression of endogenous S100a8 transcripts was determined by qRT-PCR using cDNA from different tissues of Control (n=3) and TgS100a8a9hep (n=4) mice. Mean +SD, students t-test, *p≤0.05, **p≤0.01, n.s. not significant. (B) Leukocyte subpopulations in the peripheral blood from Control and TgS100a8a9hep mice (n=4) were quantified and depicted as relative cell number. Box-plot shows median, 25 % and 75 % quartile (light and dark grey box), students t-test, **p≤0.01. (C-D) Peripheral blood from Control and TgS100a8a9hep mice (n=7) was subjected to tri-color staining using antibodies against Cd11b (APC), Gr1 (PE), and Ly-6G (FITC). Graphs show the total percentage of Gr1+/Ly-6G+ cells (C) and Gr1+/Ly-6G- cells (D) from all Cd11b+ cells in peripheral blood. Box-plot shows median, 25 % and 75 % quartile (light grey box), students t-test, *p≤0.05, n.s. not significant.