Figure 5

ASML-exosomes, immunosuppression, apoptosis and activation markers. Leukocytes were stimulated as described in Figure4. (A) Mean percent (3 experiments) of Gr1⁺, CD11b⁺ and Gr1⁺CD11b⁺ (MDSC) cells. (B) examples of CD4⁺CD25⁺, CD4⁺FoxP3⁺ and CD25⁺FoxP3⁺ cells and mean percent ± SD (3 experiments) of CD4⁺CD25⁺FoxP3⁺ cells. (C) Representative examples of AnnexinV/PI staining and mean percent ± SD (3 experiments) of AnnV-FITC⁺/AnnV-FITC⁺/PI⁺ cells. (D) Mean percent ± SD (3 experiments) of CD25⁺, CD28⁺ and CD44v6⁺ cells. (C,D) Significant differences in the presence of ASML-exosomes: *. There is no evidence for ASML-exosomes affecting MDSC or T_reg. However, apoptosis susceptibility is slightly increased and expansion of CD44v6⁺ cells is impaired.