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Figure 2 | Cell Communication and Signaling

Figure 2

From: Tumor-exosomes and leukocyte activation: an ambivalent crosstalk

Figure 2

Tumor-exosome uptake by leukocyte subpopulations. (A) LNC, SC, PBL and PEC were incubated with RhDHPE-labeled ASML-exosomes for 6 h and stained with leukocyte subset-specific antibodies. The mean percent ± SD of marker+exosome+ / marker+ cells (3 experiments) is shown. (B,C) In vivo uptake of SP-Dio18(3) labeled ASML-exosomes 24 h after i.v. application: (B) flow-cytometry of dispersed cells. The mean percent ± SD of marker+exosome+ / marker+ cells (3 rats) is shown. (C) Confocal microscopy of shock frozen tissue sections (spleen sections were stained with anti-sIgM, lymph node sections with anti-CD4, -CD8, -CD11b and -CD11c. For lymph node sections stained with anti-CD4 and anti-CD11b a higher magnification is included (scale bar spleen: 20 μm, lymph nodes: 10 μm). (A,B) Significant differences in the % exosome+marker+ cells as compared to exosome+ cells in the total organ: *. Exosomes are taken up by cells of all major leukocyte subpopulations.

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