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Figure 5 | Cell Communication and Signaling

Figure 5

From: Complex formation of EphB1/Nck/Caskin1 leads to tyrosine phosphorylation and structural changes of the Caskin1 SH3 domain

Figure 5

EphB1 phosphorylates Caskin1 on tyrosine 296 and 336 (2) . (a) Ha-EphB1 and different constructs of V5-Caskin1 were coexpressed in COS7 cells. Cell lysates were then subjected to immunoprecipitation with anti-V5 antibody. Bound proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with anti-phosphotyrosine and anti-V5 antibodies. (b) HA-EphB1 and different constructs of V5-Caskin1 were coexpressed in COS7 cells. Cell lysates were then subjected to immunoprecipitation with anti-V5 antibody. Bound proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the phosphospecific anti-PTyr296 and anti-V5 antibodies. (c) HA-EphB1 and different constructs of V5-Caskin1 were coexpressed in COS7 cells. Proteins were then immunoprecipitated with anti-V5 antibody, then samples were analyzed by the phosphospecific anti-PTyr336 and anti-V5 antibodies.

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Cell Communication and Signaling

ISSN: 1478-811X