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Figure 4 | Cell Communication and Signaling

Figure 4

From: FGF-9 accelerates epithelial invagination for ectodermal organogenesis in real time bioengineered organ manipulation

Figure 4

ECIS Assay. We chose the ECIS Z8 system, with four wells chosen as the control group, and the other four wells as the FGF-9 group. The ECIS parameters include a frequency of 15,000 Hz and impedance data were recorded every 90 s. The black line represents the control group and the pink line represents the FGF-9 group. The vertical (dash) line represents the time of medium change. (A) Before adding epithelial cells and FGF-9 (40 ng/ml), it was necessary to allow mesenchymal cells to reaggregate as a stable layer in each well of the ECIS kit. We then added 2 × 105 mesenchymal cells to each well. Impedance data showed no difference between the two groups in the first 25 h, indicating no significant proliferation or death of mesenchymal cells. (B) The invasion test began after the addition of epithelial cells and FGF-9 (40 ng/ml). We added 2 × 105 epithelial cells to each well of the 8W10E kit. We added ordinary fresh medium to the control group (black line) and fresh medium with FGF-9 (40 ng/ml) to the FGF-9 group (pink line). The vertical (Dash) line:1. Start invasion. 2–4. Change half medium in each well. 5. Stop observation at 163 h. Red arrow: significant time point (approximately 72–74 h) of impedance change in the FGF-9 group, when epithelial invagination starts to result in a thinner layer of bioengineered organ.

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