Skip to main content
Figure 5 | Cell Communication and Signaling

Figure 5

From: Role of TLR4/NADPH oxidase/ROS-activated p38 MAPK in VCAM-1 expression induced by lipopolysaccharide in human renal mesangial cells

Figure 5

LPS induces VCAM-1 expression via p38 MAPK/ATF2 in HRMCs. Cells were transfected with ATF2 siRNA or scrambled siRNA, and then incubated with LPS for (A) 8 h or (B) 4 h. The levels of VCAM-1 protein (A) and mRNA (B) expression were determined. (C) Cells were transiently transfected with VCAM-1-luc reporter gene, transfected with ATF2 siRNA or scrambled siRNA, and then incubated with LPS for 6 h. The VCAM-1 promoter activity was determined. (D) Cells were treated with LPS for the indicated time intervals. The cell lysates were subjected to Western blot using an anti-phospho-ATF2 or anti-β-actin antibody. (E) Cells were transfected with siRNA of c-Src, p47phox, p38 MAPK, or scrambled, and then treated with LPS for 90 min. The cell lysates were subjected to Western blot using an anti-phospho-ATF2 or anti-β-actin antibody. (F) Cells were pretreated with MCI-186 (100 μM) or PP1 (1 μM) for 1 h, and then treated with LPS for 90 min. Cells were fixed, labeled with an anti-ATF2 antibody, and then FITC-conjugated secondary antibody. Individual cells were imaged. All figures are representative of three independent experiments, performed in duplicate or triplicate. Data are expressed as means ± SEM. #P < 0.01, as compared with the cells exposed to scrambled siRNA alone (A, upper panel) or LPS + scrambled siRNA (A, lower panel and E). #P < 0.01, as compared with the cells exposed to LPS alone (B, C); *P < 0.05; #P < 0.01, as compared with the cells exposed to vehicle alone (D).

Back to article page