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Figure 1 | Cell Communication and Signaling

Figure 1

From: Role of TLR4/NADPH oxidase/ROS-activated p38 MAPK in VCAM-1 expression induced by lipopolysaccharide in human renal mesangial cells

Figure 1

LPS induces VCAM-1 expression via TLR4/MyD88 in HRMCs. (A) Cells were treated with LPS for the indicated time intervals. The protein levels of VCAM-1 were determined by Western blot. (B) Cells were transfected with TLR2 siRNA, TLR4 siRNA, or scrambled siRNA, and then incubated with LPS (10 μg/ml) for 8 h. The levels of TLR4, TLR2, and VCAM-1 protein were determined by Western blot. (C) Cells were transiently transfected with VCAM-1-luc reporter gene and TLR2 siRNA, TLR4 siRNA, or scrambled siRNA, and then incubated with LPS for 6 h. The VCAM-1 promoter activity was determined in the cell lysates. (D) Cells were incubated with LPS (10 μg/ml) for the indicated time intervals. The mRNA expression of TLR4 was determined by RT-PCR. (E) Cells were transfected with MyD88 siRNA or scrambled siRNA, and then incubated with LPS (10 μg/ml) for 8 h. The levels of MyD88 and VCAM-1 protein were determined by Western blot. All figures are representative of three independent experiments, performed in duplicate or triplicate. Data are expressed as means ± SEM. *P < 0.05; #P < 0.01, as compared with the cells exposed to vehicle alone (A, D). #P < 0.01, as compared with the cells exposed to scrambled siRNA alone [B (upper panel), E (upper panel)] or LPS + scrambled siRNA [B (lower panel), C, E (lower panel)].

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