Figure 5

Muscle cell conditioned media induce macrophage spreading and adhesion. Conditioned media (CM) from myoblasts treated with 0.2 mM palmitate was applied on RAW264.7 macrophages for 2 h. A) Proteins were extracted and analyzed by western blotting using specific phospho antibodies to ERK, JNK and p38 as previously described. B-C) Actin labelling with rhodamine phalloidin and cell shape quantification were performed as described in Methods. E) Circularity in images was quantified with ImageJ software. E) Measurement of RAW264.7 cell adhesion in response to CM, as described in Methods. Results were normalized to BSA control, reported as fold change ± SD and analyzed using 1-way ANOVA, n≥3, *p< 0.05 , **p<0.01.