Figure 4

Palmitate treatment does not induce insulin-resistance in muscle cells. A-B) The direct effect of PA was measured by treating L6 cells with 0.2 mM palmitate (PA), palmitoleate (PO) or BSA control for 24 h, followed by 3 h serum starvation and insulin stimulation (20 min, 100 nM) in the absence of PA. C-D) The indirect effect of palmitate through a possible autocrine effect was tested by generating conditioned media from muscle and applying it on naive untreated muscle cells. Surface GLUT4myc and glucose uptake were measured as described in Methods. Results are normalized to the CM-BSA control and expressed as mean ± SEM (n=4), **p<0.01.