Figure 3

Palmitate-induced cytokine expression occurs through NF-κB pathway. A) NF-κB was silenced using specific siRNA to the p65 subunit (p65) or control non-related siRNA (NR) before treatment of cells for 24h with 0.2 mM palmitate or BSA. B) Cells were incubated in the presence of 0.2 mM palmitate or BSA without or with N-acetyl-cysteine (5 mM) for 24 h. Inset: ROS production was measured as described in Figure 2A and reported as percent of the BSA control. All results were normalized to BSA control and reported as fold change ± SD from at least 3 independent experiments (n≥3). *p<0.05, **p<0.01, ns=non significant.