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Figure 5 | Cell Communication and Signaling

Figure 5

From: Monocytes conditioned media stimulate fibronectin expression and spreading of inflammatory breast cancer cells in three-dimensional culture: A mechanism mediated by IL-8 signaling pathway

Figure 5

IL-8 signaling pathway is involved in fibronectin production by SUM149 cells. A) Immunoblot analysis revealed that fibronectin was weakly expressed by control SUM149 cells grown in complete media (lane a). On the other hand SUM149 cells grown in media conditioned by MCP-1 and IL-8 highly express fibronectin (lanes b and c, respectively). Immunoblot analysis of PI3K-p85 revealed that control SUM149 (lane a) and SUM149 treated with MCP-1 (lane b) weakly express PI3K-p85 while SUM149 cells treated with IL-8 showed high expression of PI3K-p85. Phospho- and total Akt were analyzed by immunoblotting. Results revealed that control SUM149 (lane a) and SUM149 treated with MCP-1 (lane b) weakly express p-Akt, while SUM149 cells treated with IL-8 characterized by more expression of p-AKT (lane c). Total Akt found to be expressed by control SUM149 (lane a), SUM149 treated with MCP-1 (lane b) and SUM149 treated with IL-8 (lane c). B) Immunoblot analysis showed no alteration in expression level of IL-8 specific receptors CXCR1 and CXCR2 by control SUM149 cells grown in complete media (lane a), SUM149 cells treated with recombinant MCP-1 (lane b) and SUM149 cells treated with recombinant IL-8 (lane c). Mr. represents molecular weight marker. Results were representatives of 3 independent experiments.

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Cell Communication and Signaling

ISSN: 1478-811X