Figure 2

Cytokine profile of U937-CM. A) Human ChemiArray™ Human Inflammation Antibody Array III (Chemicon) assessed 40 cytokines, chemokines and growth factors, spots represent detected cytokines, chemokines and growth factors at different density levels. Detected low secreted cytokines were: Eotaxin (E_1,2), Eotaxin-2 (F_1,2), GCSF(G_1,2), GM-CSF (H_1,2), ICAM-1(I_1,2), IFN-γ(J_1,2), I-309 (K_1,2), IL-1α (L_1,2), IL-1β (A_3,4), IL-2 (B_3,4), IL-3(C_3,4), IL-4 (D_3,4), IL-6 (E_3,4), IL-7 (G_3,4), IL-8 (H_3,4), IL-10 (I_3,4), IL-11(J_3,4), IL-12 p 40 (K_3,4), IL-12 p 70 (L_3,4), IL-13 (A_5,6), IL-15 (B_5,6), IL-16 (C_5,6), IL-17 (D_5,6), IP-10 (E_5,6), MCP-2 (G_5,6), MIG (I_5,6), MIP-1a (J_5,6), MIP-1d (L_5,6), TGF-β (B_7,8), TNf-β (D_7,8), s TNF RI (E7,8) and PDGF-BB (G7,8). Detected moderately secreted cytokines were: IL-6 S R (f3, 4), M-CSF (H5, 6), MIP-1b (K5, 6), RANTES (A7,8), TNF-α (C7,8), s TNF RII (F7,8) and TIMP-2 (H_7,8). While, detected highly secreted cytokines were IL-8 (H_{3, 4}) and MCP-1 (F_{5, 6}). B) Bars represent dot intensities of the measured cytokines as quantified by ImageJ software. Density values 0-4000 were considered as low secreted cytokines; 4000-8000 were considered as moderately secreted cytokines, above 8000 were considered as highly secreted cytokines. Results were representatives of 3 independent experiments.