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Figure 1 | Cell Communication and Signaling

Figure 1

From: Monocytes conditioned media stimulate fibronectin expression and spreading of inflammatory breast cancer cells in three-dimensional culture: A mechanism mediated by IL-8 signaling pathway

Figure 1

Alteration in the morphology of SUM149 cells cultured in monolayer versus 3D culture models. A) SUM149 cells grown on monolayer culture for 24 h showing cells adhering to monolayer (left panel). SUM149 cells divided and became attached to each other after 48 h (middle panel) reaching 80% confluent after 72 h as shown in 9 fields' image (right panel. Nuclei were stained with blue stain DAPI. B) SUM149 cells grown in 3D overlay culture cells form aggregates after 24 h (left panel), divide and form connected like structures after 48 h (middle panel). After 72 h cell division increased and SUM149 cells formed emboli like structures which were distributed all over 3D culture model shown in 9 fields image (right panel) nuclei stained with blue stain DAPI were distributed within the tumor emboli structures, (scale bar = 20 μm). C) Archival H&E stain of paraffin embedded tissue section of IBC patient sample showing IBC cells invasion into blood and lymphatic vessels forming tumor emboli while tumor cells strongly bound together and retracted away from surrounding endothelial lining. Results were representatives of 3 independent experiments.

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Cell Communication and Signaling

ISSN: 1478-811X