Figure 1

Subcellular localization of ARTD10. A. ARTD10 interacts with the oncoprotein MYC in the nucleus. The indicated fusion proteins were transiently expressed in HeLa cells. The expression of DsRed served as a transfection control. The appearance of yellow fluorescence was monitored by confocal microscopy. B. The scheme summarizes the domain structure of ARTD10 (light green, RNA recognition motif RRM; green, glycine-rich region; yellow, glutamate-rich region; black, nuclear export sequence; blue, ubiquitin-interaction motif; red, catalytic domain). The fusions of full-length ARTD10 and of overlapping fragments of ARTD10 to CFP-YFP are indicated. These fusion proteins were transiently expressed in COS7 cells and their subcellular distribution determined in the presence or absence of Leptomycin B (LMB). The relative distribution between the cytoplasmic and the nuclear compartment is indicated for each fusion protein (for representative micrographs see Figure 2B). C. The fusion proteins indicated in panel B were expressed transiently in COS7 cells and analyzed by Western Blotting using an anti-GFP antibody. D. The subcellular localization of CFP-YFP-ARTD10(408–649) and CFP-YFP-ARTD10(408–649)ΔNES was analyzed in transiently transfected COS7 cells in the presence or absence of LMB using confocal microscopy.