Table 2 Measured affinity values
Peptide sequence | Protein/position | Expected partner | Array positive | Affinity (KD)/relative affinity (IC50) | References | Comments and other SH2 domains bound |
|---|---|---|---|---|---|---|
AEDVpYDVPPPA | p130Cas (Y362) | Abl2 | No | KD = 14 μM | This Study |  |
|  |  | Crk | Yes | KD = 0.35 μM | This Study; [87] | Fyn, Hck, Src |
|  |  | CrkL | Yes | KD = 0.99 μM | This Study |  |
|  |  | Nck1 | Yes | KD = 0.93 μM | This Study |  |
|  |  | Ptpn11_N | No | KD > 50 μM | This Study |  |
|  |  | Shc1 | No | KD = 32.8 μM | This Study |  |
|  |  | Ship2 | No | KD = 16.5 μM | This Study | Below Threshold (1.91X Mean) |
SPGEpYVNIEFG | IRS-1 (Y896) | Abl1 | No | KD = 7.48 μM | This Study | Blk Dapp1 Fgr Grb7 Itk Mist Pi3k1_N Pi3k1_C Plcg1_C Ptpn11_N Rasa1_N Rasa1_C Sh2b Sh2d2a Shb Shf Shd She Syk_C Vav1 Yes |
|  |  | Abl2 | No | KD = 3.66 μM | This Study |  |
|  |  | Crk | No | KD > 20 μM | This Study |  |
|  |  | Grb2 | Yes | KD = 0.8 μM | Gads Grap | |
|  |  |  |  | KD = 35nM |  |  |
|  |  | PI3K1_N | Yes | KD = 2.68 μM | This Study |  |
|  |  | Plcg2_C | Yes | KD = 2.92 μM | This Study |  |
|  |  | Ptpn11_N | Yes | KD = 2.08 μM | This Study |  |
|  |  |  |  | IC50 = 4.8 ± 1.0 μM |  | |
|  |  | Sh2b | Yes | KD = 3.58 μM | This Study |  |
|  |  | Src | No | KD = 2.21 μM | This Study |  |
|  |  | Tenc1 | No | KD = 24 μM | This Study |  |
NGLNpYIDLDLV | IRS-1 (Y1179) | Abl1 | No | KD = 22.2 μM | This Study | Itk Plcg1_C Rasa1_N Sh2b Shb Shf Shd She Yes |
|  |  | CrkL | No | KD > 50 μM | This Study |  |
|  |  | Grb2 | No | KD > 50 μM | This Study |  |
|  |  | PI3K1_N | No | KD = 15 μM | This Study |  |
|  |  | Ptpn11_N | Yes3 | KD = 3.0 ± 0.60nM | 3Tandem SH2 domains of Ptpn11 was used to bind to the tandem motif of IRS-1 | |
|  |  | Ptpn11_N | Yes | IC50 = 1.1 ± 0.5 μM | [107] |  |
|  |  | Src | No | KD = 9.94 μM | This Study |  |
GVSEpYELPEDP | FGFR1 (Y463) | Brk | Yes | KD = 0.38 μM | This Study |  |
|  |  | Crk | No | KD = 44 μM | This Study |  |
|  |  | CrkL | No | KD > 50 μM | This Study |  |
|  |  | Itk | Yes | KD = 2.74 μM | This Study |  |
|  |  | Nck1 | Yes | KD = 2.45 μM | This Study |  |
|  |  | Rasa1_N | No | KD = 1.54 μM | This Study |  |
|  |  | Rasa1_C | No | KD > 17 μM | This Study |  |
|  |  | Src | No | KD = 6.85 μM | This Study |  |
|  |  | Vav1 | Yes | KD = 1.87 μM | This Study |  |
STDEpYLDLSAP | FGFR3 (Y760) | Abl2 | No | KD = 27 μM | This Study |  |
|  |  | PI3K1_C | No | KD = 2.14 μM | This Study |  |
|  |  | Plcg2_N | No | KD > 50 μM | This Study |  |
|  |  | Plcg2_C | No | KD = 7.49 μM | This Study |  |
|  |  | Ptpn11_N | No | KD =25 μM | This Study |  |
|  |  | Sh2d1b | No | KD = 5.3 μM | This Study | 2.52X Mean, Lck |
|  |  | Shb | No | KD > 50 μM | This Study | Below Threshold, 0.23X mean |
|  |  | Rasa1_C | No | KD > 17 μM | This Study |  |
KEEGpYELPYNP | p62DOK1 (Y398) | Abl1 | Yes | KD = 5.77 μM | This Study | Blk Brk Fgr Fyn Hck Itk Lck Lyn Nck1 Pi3k1_N Sh2d1b Shc1 Ship2 Src Vav1 Yes |
|  |  | Abl2 | Yes | KD = 3.47 μM | This Study |  |
|  |  | Brk | Yes | KD = 0.42 μM | This Study |  |
|  |  | Crk | No | KD = 23.7 μM | This Study |  |
|  |  | Fer | No | KD = 13.3 μM | This Study |  |
|  |  | Fgr | Yes | KD = 2.04 μM | This Study |  |
|  |  | Itk | Yes | KD = 5.43 μM | This Study |  |
|  |  | Nck1 | Yes | KD = 4.37 μM | This Study |  |
|  |  | PI3K1_N | Yes | KD = 1.85 μM | This Study |  |
|  |  | PI3K1_C | No | KD = 13 μM | This Study |  |
|  |  | Ptpn11_N | No | KD > 50 μM | This Study |  |
|  |  | Rasa1_N | Yes | KD = 0.41 μM | This Study |  |
| Â | Â | Â | Â | N.A. | [123] | Â |
|  |  | Rasa1_C | Yes | KD = 1.39 μM | This Study |  |
| Â | Â | Â | Â | N.A. | [123] | Â |
|  |  | Sh3bp2 | No | KD = 1.68 μM | This Study |  |
|  |  | Shc1 | Yes | KD = 5.74 μM | This Study |  |
|  |  | Src | Yes | KD = 5.06 μM | This Study |  |
|  |  | Vav1 | Yes | KD = 4.73 μM | This Study |  |