Figure 3

High-resolution interaction maps detail an SH2 domains potential interactome. A phosphotyrosine interactome for 13 proteins involved in FGF-family and Insulin-family signaling and 50 SH2 domain partners. Phosphotyrosine peptides are indicated by their position within their host protein and color-coded as either PhosphoSite reported phosphorylation sites (yellow); sites not reported as phosphorylated (red); sites not reported to be phosphorylated but where a closely related site on a paralogous protein is known to be phosphorylated (red/yellow); or the peptide was discarded as non-specific (black). Interactions between the vertices of SH2 domains and phosphopeptides identified in this study are indicated as edges (lines) and color-coded according to the level of support provided by previous studies: if the precise phosphorylation site has been reported to interact with the noted SH2 domain the edge is denoted in red. A black line is representative of proteins that are reported to interact defined by interaction databases including HPRD, BIND, MINT and DIP, but the site of interaction is unknown. SH2 interactions not confirmed by literature but whose binding is greater than 3X mean on the array are represented with grey lines.