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Figure 1 | Cell Communication and Signaling

Figure 1

From: SRC Homology 2 Domain Binding Sites in Insulin, IGF-1 and FGF receptor mediated signaling networks reveal an extensive potential interactome

Figure 1

Probing interactions between SH2 domains and physiological peptide ligands at a systems level. (A) A representation of a SPOT peptide array containing 192 phosphotyrosine peptides including control peptides (black) and peptides from the 13 proteins present on the array indicated by their represented colors. SPOT peptide arrays were incubated with 250nM GST-SH2 domain as indicated. Interactions were detected using anti-GST antisera and Alexa-680-labeled anti-mouse secondary antibody and the intensity of signals recorded using LiCor Odyssey. (B) Neighbor-Joining Tree of all 121 SH2 domains. Highlighted in blue are the 50 SH2 domains selected across different families for this study. (C) Peptide arrays using SPOTS is a semi-quantitative method for measuring protein domain-pTyr peptide interactions. The dissociation constants (KD) were measured between 60 interaction pairs presenting interactions determined using peptide arrays as greater than 3X the mean, between 1 and 3X the mean and less than 3X the mean. The mean KD value for each group is marked with a black line.

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