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Figure 6 | Cell Communication and Signaling

Figure 6

From: Vectorial secretion of CTGF as a cell-type specific response to LPA and TGF-β in human tubular epithelial cells

Figure 6

Accumulation of intracellular CTGF upon stimulation with basolateral TGF-β. A: hPTEC were polarized and then stimulated with TGF-β for 1 h from the basolateral side. N-cadherin (green) and CTGF (red) were detected by immunocytochemistry. Control cells showed CTGF expression only in clustered proximal tubular cells (arrow, upper panels). Treatment with TGF-β from the basolateral side activated CTGF primarily in distal tubular cells (lower panels). Scale bar: 20 μm. B: Higher magnification of an experiment performed as in A with cells stimulated with TGF-β from the basolateral side. E-cadherin (red) and CTGF (green) were detected by immunocytochemistry. Arrows indicate examples of positive cells. Scale bar: 20 μm. C: Polarized hPTEC were treated with brefeldin A (10 μM) for 30 min and then were incubated with TGF-β from the apical side for 2 h. E-cadherin (red) and CTGF (green) were detected by immunocytochemistry. Arrows indicate examples of CTGF positive proximal cells, which were negative for E-cadherin; arrow heads indicate examples of CTGF positive distal cells, which weakly express E-cadherin. Scale bar: 20 μm.

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