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Figure 3 | Cell Communication and Signaling

Figure 3

From: Vectorial secretion of CTGF as a cell-type specific response to LPA and TGF-β in human tubular epithelial cells

Figure 3

Secretion of CTGF from polarized hPTEC stimulated with TGF-β. Polarized hPTEC were stimulated with TGF-β for 6 and 24 h from the basolateral side (T-b) or from the apical side (T-a). CTGF was detected in the apical (A) or basolateral (B) compartment. The blots shown are from a representative experiment performed with duplicate inserts. The graphs summarize means +/− SD of 6 (A) and 3 (B) experiments with different hPTEC isolations. Expression of control cells in each experiment was set to 1. * p < 0.05, ** p < 0.01, Dunnet’s multiple comparison test. C: RNA was isolated from polarized cells treated with TGF-β for 3 h. CTGF mRNA expression was determined by real time quantitative RT-PCR and corrected for 18 S RNA. Data are means +/− SD of duplicate determinations of duplicate inserts. RNA in control cells was set to 1. * p < 0.05, ** p < 0.01, Dunnet’s multiple comparison test.

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