Figure 1From: Vectorial secretion of CTGF as a cell-type specific response to LPA and TGF-β in human tubular epithelial cellsPolarized culture of primary human tubular epithelial cells (hPTEC). A: Freshly isolated hPTEC were cultured for 8 days in transwell inserts. Cilia were stained with acetylated tubulin and nuclei were visualized by Hoechst. For comparison, non polarized cells are shown in the right panel. Scale bar: 10 μm. B: hPTECs were cultured for 8 to 13 days in transwell inserts. Transepithelial electrical resistance (TEER) was measured in three cultures of one preparation. Data are means +/− SD.Back to article page