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Figure 5 | Cell Communication and Signaling

Figure 5

From: EGF and HB-EGF enhance the proliferation of programmable cells of monocytic origin (PCMO) through activation of MEK/ERK signaling and improve differentiation of PCMO-derived hepatocyte-like cells

Figure 5

Effect of EGF and HB-EGF treatment during PCMO culture on NeoHepatocyte function. PCMOs treated with the indicated concentrations (in μg/L) of EGF or HB-EGF were cultured in hepatocyte conditioning medium for 2 weeks before subjecting them to analysis of cytochrome P450 (CYP) isoforms 1A1/2 (A), 2D6 (B), and 3A4 (C), the phase II enzyme UDP-glucuronosyl transferase (D), urea metabolism (E), and glucose metabolism (F). Methodologic details are given in the Methods section. Data are presented as mean ± SEM of N = 4. Statistical analysis: Single-Factor ANOVA: p < 0.01 for A, B and D; ANOVA (2-Factor with replication) for E: p < 0.001 between different EGF/HB-EGF treatments; p < 0.001 between basal and NH4Cl-treatments; for F: p < 0.001 between basal and pyruvate + lactate treatments; a = significantly different vs. control; b = vs. corresponding HB-EGF value; c = vs. basal value.

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Cell Communication and Signaling

ISSN: 1478-811X