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Figure 2 | Cell Communication and Signaling

Figure 2

From: EGF and HB-EGF enhance the proliferation of programmable cells of monocytic origin (PCMO) through activation of MEK/ERK signaling and improve differentiation of PCMO-derived hepatocyte-like cells

Figure 2

EGF and HB-EGF increase the number of mitotically active cells in PCMO cultures. (A) immunofluorescence of Ki67 expression (red) in PCMO cultures treated for 4 days with the indicated concentrations of either EGF or HB-EGF. PCMOs were immunostained with CD14 (green) as a monocyte-specific marker. Nuclei were stained with DAPI (blue). (B) Quantification of Ki67 expressing PCMOs after treatment with EGF or HB-EGF. Data (N = 3) are expressed as mean ± SEM. Statistical analysis: “a” denotes a significant difference from the control; “b” denotes a significant difference from the corresponding values of HB-EGF. (C) Immunoblotting of the retinoblastoma protein and cyclin A in PCMO cultures treated for 4 days with the indicated concentrations (in μg/L) of either HB-EGF or EGF. The housekeeping protein β-actin was detected as a loading control. Densitometric analysis of cyclin A and β-actin bands was performed using NIH ImageJ software (version 1.45). The indicated values below the blots represent the normalized signal intensities for cyclin A relative to untreated control cells set at 1. (D) PCMO cell counts after 4 days of culture with 10 μg/L of either EGF or HB-EGF (N = 3). Statistical analysis: “a” denotes a significant difference from the control.

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