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Figure 1 | Cell Communication and Signaling

Figure 1

From: EGF and HB-EGF enhance the proliferation of programmable cells of monocytic origin (PCMO) through activation of MEK/ERK signaling and improve differentiation of PCMO-derived hepatocyte-like cells

Figure 1

Detection of different EGF receptor family members by RT-PCR in human blood monocytes. (A) Standard endpoint RT-PCR of EGFR (lane 2), ERBB2 (lane 3), ERBB3 (lane 4), ERBB4 (lane 5), and β-actin as control (lane 6) in PCMOs. RNA was isolated from day-4 PCMOs and reverse-transcribed. Amplification products of the resulting cDNA (using the primers listed in Table 1) were run on an agarose gel and stained with ethidiumbromide. A molecular weight marker (lane 1) was run in parallel to confirm the predicted band sizes. (B) QPCR analysis of the same EGF receptors in monocytes during the generation of PCMOs. Data shown are the mean ± SD of 3 separate experiments performed in duplicates.

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Cell Communication and Signaling

ISSN: 1478-811X