Figure 5
Involvement of PI3kinase in JNK-activation. A. Monocytes were pre-treated with Ptx (overnight incubation, 200 ng/ml) or wortmannin (2 h, 100 nM) and then stimulated with PMTwt (1 μg/ml, 4 h) and or LPS (30 ng/ml, 1 h). Whole-cell lysates were prepared, proteins were separated by SDS-PAGE and p-JNK (Thr183/Tyr185), JNK, p-AKT (Thr308) and actin were detected using specific antibodies. Shown is one representative western blot out of three. B. Cells were treated as described in A or stimulated with Ptx and wortmannin. Supernatants were collected and IL-12p40 ELISAs were performed. Results are presented as mean ± SD; n = 3. C. Monocytes were stimulated like described in B and MLRs were performed. Results are given as mean ± SD; n = 3. Statistical significance in B. and C. was calculated with a student’s t test with *P < 0.05 and **P < 0.01.