Figure 1

DUSP6 mRNA levels are elevated in cells with FLT3 ITD. mRNA expression of DUSP1, DUSP2, and DUSP6 in primary AML cells was analyzed by RT-qPCR (A) or retrieved from Affymetrix data (B). mRNA expression of the murine genes was also analyzed by RT-qPCR in 32D cells (C) and BA/F3 cells (D), either parental or expressing wildtype (WT) FLT3 or FLT3 ITD, as indicated. RT-qPCR data are based on duplicate determinations for the indicated sample numbers (A), and on duplicate or triplicate determinations in 6 (C) or 8 (D) independent experiments, and are presented as percentage of expression of the mean of 3 housekeeping genes. Values are expressed as means + SEM (A), means + 2SEM (B), or means + SD (C, D). Statistic significance was tested by Mann–Whitney test (A, B) or Students’ test (C, D) using the software SPSS (*p < 0.05, **p < 0.01). The different relative expression levels depicted in (A) versus panels (C, D) presumably relate to the different species background (human in 1A, murine in 1 C, D), including different expression levels of housekeeping genes used for reference, and use of primary patient cells (1A) versus established cell lines (1 C, D). DUSP expression levels were commonly lower in cell lines compared to primary cells (see Table 1).