Figure 6

Cytokine production and actin polymerization after CD11c stimulation. (A) Production of cytokines TNF- ∝, IL-6 and IL-10 by BMDCs of wild-type animals (WT) and ADAP-deficient animals (ADAP) was measured in the supernatants 24 h after stimulation with anti-CD11c (mean + SEM, n = 3, *P < 0.05, ns = non significant). (B) BMDCs of WT and ADAP were incubated with anti-CD11c. After incubation, cells were permeabilized and stained with TRITC-phalloidin. The cellular F-actin content was analyzed by FACS. Results are expressed as the percentage increase in mean fluorescence intensity (mean ± SEM, n = 6; P = 0.0031 for the two curves, as assessed by two-way ANOVA). (C) BMDCs of WT and ADAP were stimulated with anti-CD11c for 0, 5, 10, 20, and 30 min. Lysates were separated by SDS-PAGE, and immunoblotted with the indicated antibodies. One representative of three independent experiments is shown.