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Table 1 Measurement of Mip-1α release

From: Involvement of CD11b integrin in the alteration of metabolic factors after phorbol ester stimulation of human myeloid leukemia cells

Production and secretion of Mip-1α [ng/ml] following stimulation with 5nm TPA
  0h 4h 8h 24h 48h 72h
U937 n.d. 0.21 ± 0.16 19.75 ± 0.55 302.06 ± 22.00 331.11 ± 15.26 372.47 ± 35.47
pMTH1-U937 n.d. 0.21 ± 0.16 23.53 ± 1.66 340.45 ± 24.41 334.87 ± 7.89 344 ± 33.30
asCD11b-U937 n.d. 1.20 ± 0.11 3.06 ± 0.03 3.20 ± 0.06 2.10 ± 0.08 1.06 ± 0.15
  1. About 5x105 U937 cells, pMTH1-U937 and asCD11b-U937 cells were incubated in the absence or presence of 5nM TPA for up to 72 h, respectively. At the time points indicated aliquots from the cell supernatants were applied to a Mip-1α ELISA. The results are expressed as ng Mip-1α/ml with a detection limit below 6 pg/ml (n.d. = not detectable (below detection limit)). Data represent the mean ± s.d. of one experiment performed in triplicate.