Figure 9

Representative Ca²⁺oscillation profiles of mouse oocytes injected with lysates obtained from HEK293T cells transfected with PLCζ isoforms. Baseline values for all Ca²⁺ traces are stacked to facilitate visualization of the responses. Empty vector: Ca²⁺ trace obtained from injection of lysates obtained from HEK293T cells transfected with vector without PLCζ; PLCζ^H398P: Ca²⁺ trace obtained from injection of lysates obtained from HEK293T cells transfected with the H398P isoform of PLCζ; PLCζ^WT: Ca²⁺ trace obtained from injection of lysates obtained from HEK293T cells transfected with wild type PLCζ; CHX: cyclohexamide (an inhibitor of RNA synthesis). Figure reproduced from Kashir et al. (2011a) with permission.