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Figure 3 | Cell Communication and Signaling

Figure 3

From: Rapid alterations of cell cycle control proteins in human T lymphocytes in microgravity

Figure 3

Protein expression and phosphorylation of cdc2, cdc25 and cyclinB1 in Jurkat T cells at the onset (0 min) and after 1 min, 5 min and 10 min simulated weightlessness (μg) without (con) or with PMA (PMA) or CD3/CD28 soluble antibodies (CD3/CD28). 1g-control experiments have been performed inside the clinostat, but without rotation. Three independent experiments were performed and analysed separately by immunoblotting. Quantitation was performed by Gene Profiler software. The results are expressed as mean ± SEM. Data were analysed by one way ANOVA, followed by Bonferroni test for comparison of certain column pairs. p < 0.1 = *, p < 0.01 = **, p < 0.001 = ***

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