PDGF-induced MMP production is reduced in AdCCN5-infected cells. Uninfected VSMC or VSMC infected with AdCCN5 or AdGFP were growth arrested by serum starvation for 72 h, and then treated overnight with serum-free medium containing the indicated concentrations of PDGF-AB. Conditioned media were collected, concentrated, and run on an SDS-PAGE gelatin zymogram. The gel was stained with Coomassie Blue. The bands lacking stain indicate MMP activity. Upper band is MMP9 (Gelatinase B); lower bands are MMP2 (Gelatinase A) in both active (lower) and inactive (upper) forms as shown. Ad = Adenovirus.