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Figure 5 | Cell Communication and Signaling

Figure 5

From: Identification of a potent serum factor that causes desensitization of the receptor for C-Type natriuretic peptide

Figure 5

Sphingosine-1-phosphate rapidly desensitizes GC-B. S1P (100 nM) was added to GCB/3T3 cells at the indicated times before addition of 20 nM CNP and 5 min later the reaction was terminated with 0.5 N perchloric acid and cGMP accumulation determined as in Methods. Inset; S1P (100 nM) was added to cells for the indicated times and the levels of pERK and pAkt determined by Western blot as described in Methods.

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