Calcium signaling around Mitochondria Associated Membranes (MAMs)
© Patergnani et al; licensee BioMed Central Ltd. 2011
Received: 24 June 2011
Accepted: 22 September 2011
Published: 22 September 2011
Calcium (Ca2+) homeostasis is fundamental for cell metabolism, proliferation, differentiation, and cell death. Elevation in intracellular Ca2+ concentration is dependent either on Ca2+ influx from the extracellular space through the plasma membrane, or on Ca2+ release from intracellular Ca2+ stores, such as the endoplasmic/sarcoplasmic reticulum (ER/SR). Mitochondria are also major components of calcium signalling, capable of modulating both the amplitude and the spatio-temporal patterns of Ca2+ signals. Recent studies revealed zones of close contact between the ER and mitochondria called MAMs (Mitochondria Associated Membranes) crucial for a correct communication between the two organelles, including the selective transmission of physiological and pathological Ca2+ signals from the ER to mitochondria. In this review, we summarize the most up-to-date findings on the modulation of intracellular Ca2+ release and Ca2+ uptake mechanisms. We also explore the tight interplay between ER- and mitochondria-mediated Ca2+ signalling, covering the structural and molecular properties of the zones of close contact between these two networks.
Increase of intracellular [Ca2+] can be elicited through two fundamental mechanisms: i) Ca2+ mobilization from intracellular stores, mainly the endoplasmic reticulum (ER), or ii) entry from the extracellular milieu through the opening of plasma membrane Ca2+ channels. Mitochondria are equally important in physiological Ca2+ signalling through a process unraveled by a series of works demonstrating that Ca2+ release from the ER results in cytosolic Ca2+ increases that are paralleled by similar or even larger cycles of mitochondrial calcium uptake, and subsequent release .
Mitochondrial Ca2+ accumulation is due to the large electrochemical gradient (mitochondrial membrane potential, ψmt, usually between -150 and -180 mV). Recent studies using electron tomography techniques revealed the presence of overlapping regions between ER and mitochondria separated by a minimum distance of 10-25 nm, that allows the direct physical association of ER proteins with components of the outer mitochondrial membrane (OMM) [2, 3]. These zones were identified as 'hotspots' and have pivotal roles in several cellular functions, including an highly efficient transmission of Ca2+ from the ER to the adjacent mitochondrial network that stimulates oxidative metabolism and, conversely, might enable the metabolically energized mitochondria to regulate ER Ca2+ homeostasis .
1. Mitochondrial structure
Mitochondria, considered the "biochemical powerhouse" of the cell, are dynamic and plastic organelles, constantly subjected to remodeling, involved in a number of crucial metabolic roles, such as the tricarboxylic acid (TCA) cycle and β-oxidation of fatty acids . These organelles possess two membranes that give rise to different functional regions: the inner and outer mitochondrial membranes themselves (IMM and OMM, respectively), the cytosolic side of the OMM, the intermembrane space (IMS), and the matrix. This somewhat unusual composition enables the occurrence of a wide variety of reactions, including oxidative phosphorylation. The IMM harbours complexes of the respiratory chain, ATP synthase, and enzymes involved in heme biosynthesis [2, 6]. The OMM is also very rich in proteins. Small molecules (< 10 kDa) can pass freely between the cytoplasm and the IMS due to the presence of a number of porins. Conversely, the IMM is completely impermeable even to small molecules, including protons (with the exception of O2, CO2, and H2O). This peculiarity enables the complexes of the respiratory chain to build up a proton gradient across the IMM, required for oxidative phosphorylation. The resulting electrochemical proton gradient forms the basis of the ψmt and is utilized to generate chemical energy in the form of ATP. This ψmt is also one of the crucial factors responsible for Ca2+ uptake. Ca2+ can freely cross the OMM but in order to enter the matrix it must pass through the mitochondrial Ca2+uniporter (MCU), an IMM-located channel driven by a large electrochemical gradient [7–10].
2. ER structure
The endoplasmic reticulum (ER) is considered the largest individual intracellular organelle. It consists of a three-dimensional network of endomembranes, constituting a complex grid of microtubules and cisternae. The domains accounting for the ER are functionally and structurally distinct. Historically, ER histology describes three types of ER: smooth ER, rough ER and the nuclear envelope (NE) . This division demonstrates the individual role of each particular ER type. A morphological characterization describes the NE and peripheral ER, the later being a network of tubules and sheets reaching the most remote areas of the cell. Such structural diversity of the ER, reviewed extensively by a number of authors [12–15], is related to the variety of cellular functions played by the organelle. For such a large organelle, the ER is unexpectedly plastic . This plasticity is a crucial morphological characteristic of the ER and the remodeling ability seems to be correlated with the diversity of its functions. The most important activities of ER regard protein synthesis and maturation. Protein synthesis occurs in ribosome-rich rough ER, whereas their post-translational processing is carried out by an extensive group of chaperone proteins . Importantly, the ER acts as a transport route enabling the delivery of a number of proteins to their destination . The third function is that the ER is a dynamic reservoir of Ca2+ ions, which can be activated by both electrical and chemical cell stimulation [16, 19]. This feature renders this organelle an indispensable source of Ca2+ in many aspects of physiological signalling.
3. MAM structure
The association between the ER and mitochondria was first described by Copeland and Dalton over 50 years ago in pseudobranch gland cells . However, it was only in the beginning of the 1970s that mitochondria-ER contacts were visualized by a number of research groups [21, 22]. Further developments in microscopy techniques provided researchers with the ability to perform detailed analyses with high resolution in 3 dimensions [23, 24]. The interactions between these organelles at the contact places are so tight and strong that subcellular fractionation enabled the performance of a step through which a unique fraction, originally named as 'mitochondria-associated membranes" (MAMs fraction), can be isolated . Further developments in experimental procedures enabled scientists to isolate pure MAM fractions from yeast, different organs and tissues, as well as various cell lines [25, 26].
4. The intracellular Ca2+-signalling network
Intracellular Ca2+ signalling is versatile and fundamental for the regulation of multiple cellular processes, including development, proliferation, secretion, gene activation and cell death. The universality of Ca2+ as an intracellular messenger depends on its enormous versatility in terms of speed, amplitude, and spatio-temporal patterning. The Ca2+-signalling network can be divided into four functional units:
signalling is triggered by a stimulus that generates various Ca2+ -mobilizing signals;
the latter activate the ON mechanisms that feed Ca2+ into the cytoplasm;
Ca2+ functions as a messenger to stimulate numerous Ca2+-sensitive processes; and finally,
the OFF mechanisms, composed of pumps and exchangers, remove Ca2+ from the cytoplasm to restore the resting state .
Inside cells, Ca2+ concentration ([Ca2+]) is controlled by the simultaneous interplay of multiple counteracting processes, which can be split into "ON mechanisms" and "OFF mechanisms". With these, cells maintain a rigid control over the cytosolic level of Ca2+. In resting conditions, cells maintain a low Ca2+ concentration in the cytoplasm (around 100 nM). Ca2+ influx from the extracellular space (that posses a concentration around 1-2 mM), or Ca2+ release from intracellular Ca2+ stores, such as the ER (with concentrations of 250-600 μM) generate the intracellular calcium signals .
5. The Ca2+-induced Ca2+ release process
Cells generate their Ca2+ signals through two fundamental mechanisms that make use of internal and external sources of calcium. Ca2+-mobilizing signals from internal stores are generated by stimuli that act through a variety of cell-surface receptors, such as G-protein-linked receptors and receptor tyrosine kinases. When a ligand binds its specific receptor in the plasma membrane, the occupied receptor causes GDP-GTP exchange on an associated G-protein, Gq, that activates a specific membrane-bound phospholipase C (PLC), which in turn catalyzes the production of the two second messengers, diacylclycerol (DAG) and inositol-1,4,5-trisphosphate (IP3), by hydrolysis of phosphatidylinositol 4,5-bisphosphate in the plasma membrane. IP3, a water-soluble compound, diffuses from the plasma membrane to the endoplasmic reticulum, where it binds to specific IP3 receptors (IP3Rs) and causes Ca2+ channels within the ER to open. Sequestered Ca2+ is thus released into the cytosol, and the cytosolic [Ca2+] rises sharply to about 10 μM .
On the plasma membrane there is a large family of Ca2+ entry channels, important for the "ON reaction", which can be defined by the way in which they are activated. The most known are voltage-operated channels (VOCs) that are triggered by membrane depolarization. Other channels, called receptor-operated channels (ROCs), are sensitive to the binding of different external signals, usually transmitters. Finally, store-operated channels (SOCs), respond to the depletion of internal Ca2+ stores and contribute to the spatio-temporal pattern of Ca2+ waves .
As mentioned above, IP3Rs are the most important actors in Ca2+ release from internal stores, controlled by Ca2+ itself that acts either on the luminal or cytoplasmic sides of the channel.
IP3Rs, the main Ca2+-release channels in the ER of most cell types, consist of four subunits of about 310 kDa each with a similar general structure. In mammals, three different genes encode for three different isoforms (IP3R1, -2 and -3). Structurally, the proteins have a cytoplasmic N-terminal hydrophobic region, predicted to contain six membrane-spanning helices, and a relatively short cytoplasmic C-terminus. Functionally, the N-terminal domain contains the IP3-binding domain and a "regulatory"/"coupling" domain. IP3Rs can be modulated primarily by IP3 and Ca2+ itself; the latter can also regulate IP3Rs indirectly through calmodulin (CaM); other modulators include phosphorylation by Ca2+/CaM-dependent kinase II (CaMKII), cGMP-dependent protein kinase (PKG), protein kinase C (PKC), and cAMP-dependent protein kinase (protein kinase A, PKA). This suggests that the IP3R works as a crosstalk station between Ca2+ signalling and phosphorylation . As a confirmation of this, IP3R isoforms contain multiple phosphorylation consensus sites and many docking sites for protein kinases and phosphatases, and at least 15 different protein kinases are known to directly phosphorylate IP3R .
6. Mitochondria in calcium signalling
That mitochondria can accumulate certain ions from the suspending medium was first observed in the early 1960s, when was discovered that isolated mitochondria from rat liver, kidney, brain and heart can accumulate large net amounts of Ca2+ from the suspending medium during electron transport, up to several hundred times the initial Ca2+ content .
In these studies, initial velocities of energy-dependent Ca2+ uptake were measured by stopped-flow and dual-wavelength techniques in mitochondria isolated from hearts of rats. The first rate of Ca2+ uptake shows that the initial velocity of Ca2+ uptake was slow at low concentrations of Ca2+ and increased sigmoidally to 10 nM Ca2+/s/mg protein at 300 μM Ca2+. Similar results were obtained by the employment of mitochondria subjected of a wide range of mitochondrial protein in the medium (0.5-10 mg/ml), when these organelles were oxidizing glutamate-malate and when acetate was replacing phosphate as a permanent anion .
Comparable rates of Ca2+ uptake and sigmoidal plots were obtained with mitochondria from other mammalian hearts, as like guinea pigs, squirrels, pigeons, and frogs where the rate of Ca2+ uptake was 0.05 nM/mg/s at 5 μM Ca2+ and increased sigmoidally to 8 nM/mg/s at 200 μM Ca2+.
Mitochondrial Ca2+ uptake plays a key role in the regulation of many cells functions, ranging from ATP production to cell death. Increases in mitochondrial calcium activates several dehydrogenases and carriers, inducing an increase in the respiratory rate, H+ extrusion, and ATP production necessary for the correct energy state of the cell. However, prolonged increase in [Ca2+]m leads to the opening of the mitochondrial permeability transition pore (PTP), a critical event driving to cell death by apoptosis .
Although it is generally accepted that cellular energy metabolism, survival and death are controlled by mitochondrial calcium signals, the underlying molecular mechanisms have been completely elucidated yet. Several studies have identified three essential proteins mediating the processes of calcium influx and efflux.
6.1 Mitochondrial Calcium Uniporter (MCU)
The main transporters involved in the uptake of Ca2+ into mitochondria is the MCU, characterized by a low affinity for Ca2+; in fact, MCU takes up Ca2+ in the micromolar range and experiments in permeabilized cells report a K d of the uniporter of 10 μM . In addition, a biphasic effect of calcium on the MCU has been reported: beyond a certain level, cytosolic Ca2+ inactivates the uniporter, preventing further Ca2+ uptake and this process might avoid an excessive accumulation of the cation in mitochondria .
In spite of repeated efforts by different researchers, the molecular identity of the MCU has remained elusive. Among the early candidates proposed for the MCU were the uncoupling proteins UCP2/3 , but experiments in different tissues of mice lacking UCP2 and UCP3 showed a normal Ca2+ uptake . Recently, Perocchi and colleagues  demonstrated that MICU1 (mitochondrial calcium uptake 1), also known as FLJ12684 or CBARA1, has a key role in regulating the classically defined uniporter. MICU1 is associated with the IMM and has two canonical EF hands that are essential for its activity and it caused a significant suppression of the [Ca2+]m signal evoked by an IP3-linked agonist. Silencing MICU1 does not impair mitochondrial respiration or membrane potential but abolishes Ca2+ entry in intact and permeabilized cells, and attenuates the metabolic coupling between cytosolic Ca2+ transients and activation of matrix dehydrogenases.
More recently, in 2011, two distinct laboratories have been identified a transmembrane protein (CCDC109A) that fulfilling the criteria for being the MCU [9, 10]. Indeed, in planar lipid bilayers CCDC109A showed channel activity with electrophysiological properties as those previously reported for the MCU . The over-expression of CCDC109A (that now is called "MCU"), increases mitochondrial Ca2+ uptake and sensitizes cells to apoptotic stimuli, and the employment of short interfering RNA (siRNA) silencing of MCU strongly reduced mitochondrial Ca2+ uptake. This reduction is specific for mitochondria (Ca2+ cytosolic levels remain almost unaffected), does not induce impairment of the electrochemical gradient or change in mitochondrial morphology and the induction of specific mutations at the level of the putative pore-forming region reduce the mitochondrial calcium uptake and blocks the channel activity of the protein [9, 10].
To conclude, MCU and MICU1 are critical for the correct mitochondrial calcium uptake: the first one can be considered the main component of the uniporter, while MICU1 as a fundamental regulator.
As described above, MCU only takes up Ca2+ in the micromolar range, but evidence has shown that mitochondria are able to take up Ca2+ also at much lower concentrations, as recently reported by Jang and colleagues who identified a high-affinity mitochondrial Ca2+/H+ exchanger capable of importing calcium in the nanomolar range . This group conducted a genome-wide RNAi screen in Drosophila cells stably expressing a mitochondria-targeted ratiometric Pericam and identified the gene CG4589 (Drosophila homolog of the human gene LETM1, leucine zipper-EF-hand containing transmembrane protein 1) as a regulator of mitochondrial Ca2+ and H+ concentrations, supporting electrogenic import of Ca2+ (one Ca2+ in for one H+ out).
However, the effective role of LETM1 as Ca2+/H+ exchanger still remains a subject of discussion, since its activity is blocked by treatment with CGP37157 (channel inhibitor that mediates mitochondrial calcium efflux) and red/Ru360 (inhibitor of MCU). Furthermore, LETM1 is associated with K+ homeostasis, and the loss of LETM1 lowers mitochondrial membrane potential, and the mitochondrial H+/Ca2+ exchanger turned out to be non-electrogenic (one Ca2+ in for two H+ out) [45, 46].
A Na+-dependent mechanism that mediates mitochondrial Ca2+ efflux has been demonstrated, but the molecular identity of this transporter has also remained elusive. In a recent study, Palty and co-workers showed that the Na+/Ca2+ exchanger NCLX is enriched in mitochondria, where it is localized to the cristae . This protein was identified as a member of the Na+/Ca2+ exchanger situated in the ER or plasma membrane, but Palty et al., shown that in several tissues endogenous NCLX is enriched primarily in mitochondria, but not in ER and plasma membrane. The same observation is achieved overexpressing the protein in different cell lines, and the results show that expression of NCLX enhances mitochondrial Ca2+ efflux; this is blocked by CGP37157 and by mutations in the catalytic site of NCLX. Besides, the role of NCLX as a mitochondrial Na+/Ca2+ exchanger is supported by evidence that NCLX mediates Li+/Ca2+ exchange, a functional property that, among NCX proteins, is shared exclusively with the mitochondrial exchanger .
7. Intracellular calcium extrusion mechanism
Once it activates its downstream targets, Ca2+ has carried out its functions and needs to be rapidly removed from the cytosol to restore the resting levels of approximately 100 nM. For this purpose, the cell uses the combined activity of Ca2+ extrusion mechanisms (such as PMCA and NCX) and mechanisms that refill the intracellular stores (like sarco-endoplasmic reticulum Ca2+-ATPases, SERCAs, and the secretory-pathway Ca2+-ATPases, SPCAs, of the Golgi apparatus).
The plasma membrane calcium ATPases (PMCA) is localized on the plasma membrane and couples ATP hydrolysis to the maintenance of appropriate cytoplasmic calcium levels by removing calcium from the cytosol to the extracellular spaces. There are at least four different PMCA isoforms (PMCA1-4) and several splice variants (about 26) that are encoded by four independent genes. Some of these are ubiquitously expressed in the organism (PMCA1 and -4), while others (PMCA2 and -3) have a tissue-specific expression patterns. Structurally, PMCAs consist of 10 transmembrane domains, two major intracellular loops, and N- and C-cytoplasmic domains. The pump operates with high Ca2+ affinity and low transport capacity, with a 1:1 Ca2+/ATP stoichiometry. Under optimal conditions, the K d of PMCA for Ca2+ is about 10-30 μM in resting conditions and about 0.2-0.5 μM in activated conditions .
It has been demonstrated that PMCA operates as a Ca2+/H+ exchanger and, even if the exact stoichiometry is not well defined, a recent study suggests that the Ca2+:H+ ratio is 1:2 and that the activity of the pump is insensitive to variations of membrane potential .
NCX (Na2+/Ca2+ exchanger) is a plasma-membrane enzyme, mainly located in excitable tissue, which carries out the efflux of one Ca2+ against an influx of 3 Na+. NCX easily reverses its direction and brings Ca2+ into the cells if the Na+ concentration gradient decreases and/or the membrane potential becomes less negative. Three distinct genes are known to encode as many isoforms, namely NCX1, NCX2 and NCX3 . The first one has an ubiquitous distribution, while NCX2 is expressed primarily in the brain, and NCX3 in the skeletal muscle. The NCX1 protein contains 11 putative transmembrane domains, divided into two sets of putative transmembrane domains separated by a large intracellular loop mainly responsible for the transport of the Na+ and Ca2+ across the membrane .
SPCAs are the newest addition to the family of phosphorylation-type ATPases and they are responsible for supplying the lumen of the Golgi apparatus with Ca2+. Unlike other Ca2+-ATPase pumps, SPCA pumps are not electrogenic; in fact, they do not counter-transport H+ to the outside since protons are essential for the correct development and functioning of the Golgi vesicle . In addition to Ca2+ transport, the most important property of SPCA pumps is also to transport efficiently Mn2+ into the Golgi, as this is a necessary cation for enzymes present in the lumen of the Golgi compartment. SPCAs function through a reversibly cycle between an E1- and an E2-conformation. In the cytosol, the high-affinity binding site of the protein in E1-conformation binds Ca2+ (or Mn2+), and phosphorylation by ATP creates an high-energy phosphoenzyme intermediate. This enzyme undergoes a rate-limiting transition to the lower-energy state, E2, and simultaneously Ca2+ (or Mn2+) moves through the transmembrane pore and is released into the lumen of the Golgi apparatus. As a last step, the protein returns to the dephosphorylated state .
SERCA is a pump identified in 1961-1962 in a skeletal muscle fraction. It is localized in the membranes of endo(sarco)plasmic reticulum and couples ATP hydrolysis to the transport of Ca2+ from cytoplasm to lumen. Early studies shown that the pump counter-transported H+ in exchange for two Ca2+ per ATP hydrolyzed. However, it has been noticed that fewer than four H+ were released to the cytosol per two Ca2+ pumped, showing that the transport reaction was only partly electrogenic . Like other Ca2+-ATPase pumps, SERCAs exist in two conformational states. The E1 on the cytosolic site, in which the enzyme has high Ca2+ affinity, and the E2 state, in which the lower Ca2+ affinity leads to the release of Ca2+ on the opposite side. This cycle has a number of other states that occur upon binding of Ca2+, involving a series of structural changes in the cytoplasmic sector and in the transmembrane domain, necessary for completing the catalytic cycle. The peculiarity of SERCAs in respect to the other Ca2+-ATPase pumps is to have two Ca2+ binding sites, enabling the existence of a Ca2+/ATP transport stoichiometry of 2.0 .
8. MAMs, a functional link between ER and mitochondria
As described above, mitochondria and endoplasmic reticulum networks are fundamental for the maintenance of calcium homeostasis. Recently, different studies have documented the crucial role that MAMs play in intracellular Ca2+ signalling. The physical proximity of the ER to mitochondria enables a direct, selective transmission of physiological and pathological Ca2+ signals , an aspect highly variable between cell types. In fact, mitochondria are not always morphologically continuous, functionally homogenous and associated to ER. At demonstration of this, different works revealed the existence of a largely interconnected mitochondria network akin to ER in HeLa cells, COS-7 cells, cardiac myocytes and rat hepatocytes [24, 55]. Contrary, it has been also reported that mitochondria can exist as two distinct populations, one in perinuclear position and the other one in cell periphery, with different biochemical and respiratory properties . Or, again, mitochondria within individual cells are morphologically heterogeneous and appear as distinct entities . These different aspects could carry out diverse aspect of mitochondrial functions, in particular Ca2+ sequestration, fundamental for the regulation of mitochondrial metabolism and regulation of apoptosis . Lately, it has been demonstrated that the juxtaposition between ER and mitochondria is also regulated by cellular status. In fact, a condition of starvation (an autophagic trigger) leads to PKA activation, which in turn phosphorylates the pro-fission dynamin-related protein 1 (DRP1) with consequent mitochondria elongation in a network of highly interconnected organelles. This mitochondrial elongation protects cells from death and is required to sustain ATP levels and viability .
MAMs proteins involved in ER-mitochondria Ca2+ cross-talk and relative functions
protein kinase B
Ca2+ signaling, apoptosis
Protein phosphatase 2
Ca2+ signaling, apoptosis
adenine nucleotide translocase
Part of mitochondrial contact sites and/or PTP
B-cell receptor-associated protein 31
Ca2+ signaling, apoptosis
endoplasmic reticulum resident protein 44
glucose-regulated protein 75
78 kDa glucose-regulated protein
inositol 1,4,5-triphosphate receptor
the 66 kDa isoform of ShcA protein
ROS production and signal transduction
phosphofurin acidic cluster sorting protein 2
Protein sorting, Ca2+ handling
phosphatidylethanolamine N-methyltransferase 2
promyelocytic leukemia protein
PSS-1 and -2
phosphatidylserine synthase 1 and 2
sarcoplasmic reticulum calcium ATPase 2b
truncated sarco(endo)plasmic reticulum Ca2+ ATPase
Ca2+ leak from ER
voltage-dependent anion channel
Chanel, Ca2+ handling
S100 calcium binding protein B
apoE, apoB and apoC
58 kDa protein
B-cell lymphoma 2 protein
Ca2+ homeostasis, apoptosis
B-cell lymphoma-extra large protein
Ca2+ homeostasis, apoptosis
MFN 1 and 2
Mitofuzin 1 and 2
TIM & TOM complexes
Transporter Inner Membrane complex & Transporter Outer Membrane complex
The human cytomegalovirus
p7 and NS5B protein
proteins of hepatitis C virus
acyl- CoA:cholesterol acyltransferase
ER oxidase 1 alpha
Recently identified, the Sigma-1 ER receptor (Sig-1R) selectively resides at the MAMs, forms a Ca2+-sensitive chaperone complex with BiP/GRP78 (78-kDa glucose-regulated protein GRP78, also referred to as the immunoglobulin binding protein BiP) and associates with isoform 3 of IP3R. Upon activation of IP3Rs, which causes the decrease of Ca2+ concentration at the MAM, redistribution of Sig-1Rs occurs, from MAMs to the periphery of the ER: here Sig-1Rs dissociates from BiP/GRP78 and the chaperone activity of free Sig-1Rs attenuates the aggregation of IP3R3 .
Obviously, other proteins that are required to modulate calcium mobilization upon cellular stimulation are directed to MAMs. An example is the anti-apoptotic protein AKT/PKB that, in response to survival signals, is recruited to MAMs in order to inactivate IP3R3, significantly reducing ER-Ca2+ release activity with a diminished cellular sensitivity to apoptotic stimuli . In turn, this event determines the PML (promyelocytic leukemia protein)-mediated recruitment of phosphatase PP2a (protein phosphatase 2a) at the MAMs to switch off the kinase. Also cytochrome c, which is released from mitochondria upon activation of apoptotic pathways, can bind IP3Rs at the MAMs, further activating the Ca2+ flux and enhancing apoptotic signaling . Recently, two interesting proteins with a marked regulatory effect on cell survival through changes in Ca2+ have been identified in the zones of mitochondria-ER association.
p66Shc (a 66-kDa isoform of the growth factor adapter Shc) is a cytosolic adaptor protein, profoundly involved in the cellular response to oxidative stress. This protein has also been found in the MAM fraction. Its direct relation to mitochondrial ROS production has been repeatedly documented, also by our groups [66–68]. We found that the level of p66Shc in the MAM fraction is age-dependent and corresponds well to the mitochondrial ROS production which is found to increase with age .
In this review, we have sketched out the main features of the intracellular Ca2+-signalling toolkit and the elaborate relationship between ER and mitochondria. It has been demonstrated that the physical interactions between ER and mitochondria, known as MAMs, are essential for functions of the two organelles, and that these also enable an highly efficient transmission of Ca2+ from the ER to mitochondria. In this regard, it becomes evident that the ER-mitochondria interface points deeply affect intracellular Ca2+ signalling and are fundamental for different functional outcomes, such as cell metabolism or induction of cell death.
List of abbreviations
mitochondrial membrane potential
Protein kinase B
Binding immunoglobulin Protein/78-kDa glucose-regulated protein
cytosolic Ca2+ concentration
mitochondrial Ca2+ concentration
calmodulin-dependent protein kinase II
dynamin-related protein 1
long-chain fatty acid-CoA ligase type 4
glucose-regulated protein 75
inner mitochondrial membrane
inositol 1,4,5-trisphosphate receptor
leucine zipper-EF-hand containing transmembrane protein 1
mitochondrial Ca2+ uniporter
mitochondrial calcium uptake 1
outer mitochondrial membrane
66-kDa isoform of the growth factor adapter shc
protein kinase A
protein kinase C
cGMP-dependent protein kinase
plasma membrane Ca2+ ATPase
promyelocytic leukemia protein
protein phosphatase 2a
permeability transition pore
receptor operated Ca2+ channels
reactive oxygen species
sarco-endoplasmic reticulum Ca2+ ATPase
second messenger operated Ca2+ channels
voltage-dependent anion channel
voltage operated Ca2+ channels.
This research was supported by: the Ministry of Science and Higher Education, Poland, grants N301 092 32/3407, N407 075 137, by the Polish Mitochondrial Network for MRW, JD and JS.
And by the Italian Association for Cancer Research (AIRC), Telethon (GGP09128), local funds from the University of Ferrara, the Italian Ministry of Education, University and Research (COFIN), the Italian Cystic Fibrosis Research Foundation and Italian Ministry of Health to P.P.
SM was supported by a FIRC fellowship; AB was supported by a research fellowship FISM - Fondazione Italiana Sclerosi Multipla - Cod. 2010/B/1; SP was supported by a training fellowship FISM - Fondazione Italiana Sclerosi Multipla - Cod. 2010/B/13; JS was supported by PhD fellowship from The Foundation for Polish Science (FNP), UE, European Regional Development Fund and Operational Programme "Innovative economy".
- Giorgi C, De Stefani D, Bononi A, Rizzuto R, Pinton P: Structural and functional link between the mitochondrial network and the endoplasmic reticulum. Int J Biochem Cell Biol. 2009, 41 (10): 1817-27. 10.1016/j.biocel.2009.04.010.PubMed CentralView ArticlePubMedGoogle Scholar
- Fernie AR, Carrari F, Sweetlove LJ: Respiratory metabolism: glycolysis, the TCA cycle and mitochondrial electron transport. Curr Opin Plant Biol. 2004, 7 (3): 254-61. 10.1016/j.pbi.2004.03.007.View ArticlePubMedGoogle Scholar
- Rizzuto R, Duchen MR, Pozzan T: Flirting in little space: the ER/mitochondria Ca2+ liaison. Sci STKE. 2004, 2004 (215): re1-Google Scholar
- Csordás G, Renken C, Várnai P, Walter L, Weaver D, Buttle KF, Balla T, Mannella CA, Hajnóczky G: Structural and functional features and significance of the physical linkage between ER and mitochondria. J Cell Biol. 2006, 174 (7): 915-21. 10.1083/jcb.200604016.PubMed CentralView ArticlePubMedGoogle Scholar
- McBride HM, Neuspiel M, Wasiak S: Mitochondria: more than just a powerhouse. Curr Biol. 2006, 16 (14): R551-60. 10.1016/j.cub.2006.06.054.View ArticlePubMedGoogle Scholar
- Rich PR: The molecular machinery of Keilin's respiratory chain. Biochem Soc Trans. 2003, 31 (Pt 6): 1095-105.View ArticlePubMedGoogle Scholar
- Nicholls DG, Crompton M: Mitochondrial calcium transport. FEBS Lett. 1980, 111 (2): 261-8. 10.1016/0014-5793(80)80806-4.View ArticlePubMedGoogle Scholar
- Kirichok Y, Krapivinsky G, Clapham DE: The mitochondrial calcium uniporter is a highly selective ion channel. Nature. 2004, 427 (6972): 360-4. 10.1038/nature02246.View ArticlePubMedGoogle Scholar
- De Stefani D, Raffaello A, Teardo E, Szabò I, Rizzuto R: A forty-kilodalton protein of the inner membrane is the mitochondrial calcium uniporter. Nature. 2011Google Scholar
- Baughman JM, Perocchi F, Girgis HS, Plovanich M, Belcher-Timme CA, Sancak Y, Bao XR, Strittmatter L, Goldberger O, Bogorad RL, Koteliansky V, Mootha VK: Integrative genomics identifies MCU as an essential component of the mitochondrial calcium uniporter. Nature. 2011Google Scholar
- Baumann O, Walz B: Endoplasmic reticulum of animal cells and its organization into structural and functional domains. Int Rev Cytol. 2001, 205: 149-214.View ArticlePubMedGoogle Scholar
- Voeltz GK, Prinz WA, Shibata Y, Rist JM, Rapoport TA: A class of membrane proteins shaping the tubular endoplasmic reticulum. Cell. 2006, 124 (3): 573-86. 10.1016/j.cell.2005.11.047.View ArticlePubMedGoogle Scholar
- Shibata Y, Voeltz GK, Rapoport TA: Rough sheets and smooth tubules. Cell. 2006, 126 (3): 435-9. 10.1016/j.cell.2006.07.019.View ArticlePubMedGoogle Scholar
- English AR, Zurek N, Voeltz GK: Peripheral ER structure and function. Curr Opin Cell Biol. 2009, 21 (4): 596-602. 10.1016/j.ceb.2009.04.004.PubMed CentralView ArticlePubMedGoogle Scholar
- Shibata Y, Shemesh T, Prinz WA, Palazzo AF, Kozlov MM, Rapoport TA: Mechanisms determining the morphology of the peripheral ER. Cell. 2010, 143 (5): 774-88. 10.1016/j.cell.2010.11.007.PubMed CentralView ArticlePubMedGoogle Scholar
- Bootman MD, Petersen OH, Verkhratsky A: The endoplasmic reticulum is a focal point for co-ordination of cellular activity. Cell Calcium. 2002, 32 (5-6): 231-4. 10.1016/S0143416002002002.View ArticlePubMedGoogle Scholar
- Chevet E, Cameron PH, Pelletier MF, Thomas DY, Bergeron JJ: The endoplasmic reticulum: integration of protein folding, quality control, signaling and degradation. Curr Opin Struct Biol. 2001, 11 (1): 120-4. 10.1016/S0959-440X(00)00168-8.View ArticlePubMedGoogle Scholar
- Palade G: Intracellular aspects of the process of protein synthesis. Science. 1975, 189 (4200): 347-58. 10.1126/science.1096303.View ArticlePubMedGoogle Scholar
- Verkhratsky A, Petersen OH: The endoplasmic reticulum as an integrating signalling organelle: from neuronal signalling to neuronal death. Eur J Pharmacol. 2002, 447 (2-3): 141-54. 10.1016/S0014-2999(02)01838-1.View ArticlePubMedGoogle Scholar
- Copeland DE, Dalton AJ: An association between mitochondria and the endoplasmic reticulum in cells of the pseudobranch gland of a teleost. J Biophys Biochem Cytol. 1959, 5 (3): 393-6. 10.1083/jcb.5.3.393.PubMed CentralView ArticlePubMedGoogle Scholar
- Lewis JA, Tata JR: A rapidly sedimenting fraction of rat liver endoplasmic reticulum. J Cell Sci. 1973, 13 (2): 447-59.PubMedGoogle Scholar
- Morre DJ, Merritt WD, Lembi CA: Connections between mitochondria and endoplasmic reticulum in rat liver and onion stem. Protoplasma. 1971, 73 (1): 43-9. 10.1007/BF01286410.View ArticlePubMedGoogle Scholar
- de Brito OM, Scorrano L: Mitofusin 2 tethers endoplasmic reticulum to mitochondria. Nature. 2008, 456 (7222): 605-10. 10.1038/nature07534.View ArticlePubMedGoogle Scholar
- Rizzuto R, Pinton P, Carrington W, Fay FS, Fogarty KE, Lifshitz LM, Tuft RA, Pozzan T: Close contacts with the endoplasmic reticulum as determinants of mitochondrial Ca2+ responses. Science. 1998, 280 (5370): 1763-6. 10.1126/science.280.5370.1763.View ArticlePubMedGoogle Scholar
- Vance JE, Stone SJ, Faust JR: Abnormalities in mitochondria-associated membranes and phospholipid biosynthetic enzymes in the mnd/mnd mouse model of neuronal ceroid lipofuscinosis. Biochim Biophys Acta. 1997, 1344 (3): 286-99.View ArticlePubMedGoogle Scholar
- Wieckowski MR, Giorgi C, Lebiedzinska M, Duszynski J, Pinton P: Isolation of mitochondria-associated membranes and mitochondria from animal tissues and cells. Nat Protoc. 2009, 4 (11): 1582-90. 10.1038/nprot.2009.151.View ArticlePubMedGoogle Scholar
- Berridge MJ, Lipp P, Bootman MD: The versatility and universality of calcium signalling. Nat Rev Mol Cell Biol. 2000, 1 (1): 11-21.View ArticlePubMedGoogle Scholar
- Demaurex N, Frieden M: Measurements of the free luminal ER Ca2+ concentration with targeted "cameleon" fluorescent proteins. Cell Calcium. 2003, 34 (2): 109-19. 10.1016/S0143-4160(03)00081-2.View ArticlePubMedGoogle Scholar
- Berridge MJ, Bootman MD, Roderick HL: Calcium signalling: dynamics, homeostasis and remodelling. Nat Rev Mol Cell Biol. 2003, 4 (7): 517-29. 10.1038/nrm1155.View ArticlePubMedGoogle Scholar
- Balla T: Regulation of Ca2+ entry by inositol lipids in mammalian cells by multiple mechanisms. Cell Calcium. 2009, 45 (6): 527-34. 10.1016/j.ceca.2009.03.013.PubMed CentralView ArticlePubMedGoogle Scholar
- Carafoli E, Santella L, Branca D, Brini M: Generation, control, and processing of cellular calcium signals. Crit Rev Biochem Mol Biol. 2001, 36 (2): 107-260. 10.1080/20014091074183.View ArticlePubMedGoogle Scholar
- Vanderheyden V, Devogelaere B, Missiaen L, De Smedt H, Bultynck G, Parys JB: Regulation of inositol 1,4,5-trisphosphate-induced Ca2+ release by reversible phosphorylation and dephosphorylation. Biochim Biophys Acta. 2009, 1793 (6): 959-70. 10.1016/j.bbamcr.2008.12.003.PubMed CentralView ArticlePubMedGoogle Scholar
- Budd SL, Nicholls DG: A reevaluation of the role of mitochondria in neuronal Ca2+ homeostasis. J Neurochem. 1996, 66 (1): 403-11.View ArticlePubMedGoogle Scholar
- Duchen MR: Contributions of mitochondria to animal physiology: from homeostatic sensor to calcium signalling and cell death. J Physiol. 1999, 516 (Pt 1): 1-17.PubMed CentralView ArticlePubMedGoogle Scholar
- Jouaville LS, Ichas F, Holmuhamedov EL, Camacho P, Lechleiter JD: Synchronization of calcium waves by mitochondrial substrates in Xenopus laevis oocytes. Nature. 1995, 377 (6548): 438-41. 10.1038/377438a0.View ArticlePubMedGoogle Scholar
- Rossi CS, Lehninger AL: Stoichiometric relationships between mitochondrialion accumulation and oxidative phosphorylation. Biochem Biophys Res Commun. 1963, 11: 441-6. 10.1016/0006-291X(63)90089-5.View ArticlePubMedGoogle Scholar
- Scarpa A, Graziotti P: Mechanisms for intracellular calcium regulation in heart. I. Stopped-flow measurements of Ca2+ uptake by cardiac mitochondria. J Gen Physiol. 1973, 62 (6): 756-72. 10.1085/jgp.62.6.756.PubMed CentralView ArticlePubMedGoogle Scholar
- Lehninger AL: Mitochondria and calcium ion transport. Biochem J. 1970, 119 (2): 129-38.PubMed CentralView ArticlePubMedGoogle Scholar
- Giorgi C, Romagnoli A, Pinton P, Rizzuto R: Ca2+ signaling, mitochondria and cell death. Curr Mol Med. 2008, 8 (2): 119-30. 10.2174/156652408783769571.View ArticlePubMedGoogle Scholar
- Bragadin M, Pozzan T, Azzone GF: Kinetics of Ca2+ carrier in rat liver mitochondria. Biochemistry. 1979, 18 (26): 5972-8. 10.1021/bi00593a033.View ArticlePubMedGoogle Scholar
- Moreau B, Nelson C, Parekh AB: Biphasic regulation of mitochondrial Ca2+ uptake by cytosolic Ca2+ concentration. Curr Biol. 2006, 16 (16): 1672-7. 10.1016/j.cub.2006.06.059.View ArticlePubMedGoogle Scholar
- Trenker M, Malli R, Fertschai I, Levak-Frank S, Graier WF: Uncoupling proteins 2 and 3 are fundamental for mitochondrial Ca2+ uniport. Nat Cell Biol. 2007, 9 (4): 445-52. 10.1038/ncb1556.PubMed CentralView ArticlePubMedGoogle Scholar
- Brookes PS, Parker N, Buckingham JA, Vidal-Puig A, Halestrap AP, Gunter TE, Nicholls DG, Bernardi P, Lemasters JJ, Brand MD: UCPs--unlikely calcium porters. Nat Cell Biol. 2008, 10 (11): 1235-7. 10.1038/ncb1108-1235. author reply 1237-40PubMed CentralView ArticlePubMedGoogle Scholar
- Perocchi F, Gohil VM, Girgis HS, Bao XR, McCombs JE, Palmer AE, Mootha VK: MICU1 encodes a mitochondrial EF hand protein required for Ca2+ uptake. Nature. 2010, 467 (7313): 291-6. 10.1038/nature09358.PubMed CentralView ArticlePubMedGoogle Scholar
- Jiang D, Zhao L, Clapham DE: Genome-wide RNAi screen identifies Letm1 as a mitochondrial Ca2+/H+ antiporter. Science. 2009, 326 (5949): 144-7. 10.1126/science.1175145.PubMed CentralView ArticlePubMedGoogle Scholar
- Nowikovsky K, Froschauer EM, Zsurka G, Samaj J, Reipert S, Kolisek M, Wiesenberger G, Schweyen RJ: The LETM1/YOL027 gene family encodes a factor of the mitochondrial K+ homeostasis with a potential role in the Wolf-Hirschhorn syndrome. J Biol Chem. 2004, 279 (29): 30307-15. 10.1074/jbc.M403607200.View ArticlePubMedGoogle Scholar
- Palty R, Silverman WF, Hershfinkel M, Caporale T, Sensi SL, Parnis J, Nolte C, Fishman D, Shoshan-Barmatz V, Herrmann S, Khananshvili D, Sekler I: NCLX is an essential component of mitochondrial Na+/Ca2+ exchange. Proc Natl Acad Sci USA. 2010, 107 (1): 436-41. 10.1073/pnas.0908099107.PubMed CentralView ArticlePubMedGoogle Scholar
- Brini M, Carafoli E: Calcium pumps in health and disease. Physiol Rev. 2009, 89 (4): 1341-78. 10.1152/physrev.00032.2008.View ArticlePubMedGoogle Scholar
- Thomas RC: The plasma membrane calcium ATPase (PMCA) of neurones is electroneutral and exchanges 2 H+ for each Ca2+ or Ba2+ ion extruded. J Physiol. 2009, 587 (Pt 2): 315-27.PubMed CentralView ArticlePubMedGoogle Scholar
- Philipson KD, Nicoll DA: Sodium-calcium exchange: a molecular perspective. Annu Rev Physiol. 2000, 62: 111-33. 10.1146/annurev.physiol.62.1.111.View ArticlePubMedGoogle Scholar
- Van Baelen K, Vanoevelen J, Missiaen L, Raeymaekers L, Wuytack F: The Golgi PMR1 P-type ATPase of Caenorhabditis elegans. Identification of the gene and demonstration of calcium and manganese transport. J Biol Chem. 2001, 276 (14): 10683-91. 10.1074/jbc.M010553200.View ArticlePubMedGoogle Scholar
- Dode L, Andersen JP, Raeymaekers L, Missiaen L, Vilsen B, Wuytack F: Functional comparison between secretory pathway Ca2+/Mn2+-ATPase (SPCA) 1 and sarcoplasmic reticulum Ca2+-ATPase (SERCA) 1 isoforms by steady-state and transient kinetic analyses. J Biol Chem. 2005, 280 (47): 39124-34. 10.1074/jbc.M506181200.View ArticlePubMedGoogle Scholar
- Yu X, Carroll S, Rigaud JL, Inesi G: H+ countertransport and electrogenicity of the sarcoplasmic reticulum Ca2+ pump in reconstituted proteoliposomes. Biophys J. 1993, 64 (4): 1232-42. 10.1016/S0006-3495(93)81489-9.PubMed CentralView ArticlePubMedGoogle Scholar
- Toyoshima C: Structural aspects of ion pumping by Ca2+-ATPase of sarcoplasmic reticulum. Arch Biochem Biophys. 2008, 476 (1): 3-11. 10.1016/j.abb.2008.04.017.View ArticlePubMedGoogle Scholar
- De Giorgi F, Lartigue L, Ichas F: Electrical coupling and plasticity of the mitochondrial network. Cell Calcium. 2000, 28 (5-6): 365-70. 10.1054/ceca.2000.0177.View ArticlePubMedGoogle Scholar
- Lombardi A, Damon M, Vincent A, Goglia F, Herpin P: Characterisation of oxidative phosphorylation in skeletal muscle mitochondria subpopulations in pig: a study using top-down elasticity analysis. FEBS Lett. 2000, 475 (2): 84-8. 10.1016/S0014-5793(00)01633-1.View ArticlePubMedGoogle Scholar
- Collins TJ, Berridge MJ, Lipp P, Bootman MD: Mitochondria are morphologically and functionally heterogeneous within cells. EMBO J. 2002, 21 (7): 1616-27. 10.1093/emboj/21.7.1616.PubMed CentralView ArticlePubMedGoogle Scholar
- Gomes LC, Di Benedetto G, Scorrano L: During autophagy mitochondria elongate, are spared from degradation and sustain cell viability. Nat Cell Biol. 2011, 13 (5): 589-98. 10.1038/ncb2220.PubMed CentralView ArticlePubMedGoogle Scholar
- Pinton P, Giorgi C, Siviero R, Zecchini E, Rizzuto R: Calcium and apoptosis: ER-mitochondria Ca2+ transfer in the control of apoptosis. Oncogene. 2008, 27 (50): 6407-18. 10.1038/onc.2008.308.PubMed CentralView ArticlePubMedGoogle Scholar
- Rimessi A, Giorgi C, Pinton P, Rizzuto R: The versatility of mitochondrial calcium signals: from stimulation of cell metabolism to induction of cell death. Biochim Biophys Acta. 2008, 1777 (7-8): 808-16. 10.1016/j.bbabio.2008.05.449.PubMed CentralView ArticlePubMedGoogle Scholar
- Rizzuto R, Brini M, Murgia M, Pozzan T: Microdomains with high Ca2+ close to IP3-sensitive channels that are sensed by neighboring mitochondria. Science. 1993, 262 (5134): 744-7. 10.1126/science.8235595.View ArticlePubMedGoogle Scholar
- Szabadkai G, Bianchi K, Várnai P, De Stefani D, Wieckowski MR, Cavagna D, Nagy AI, Balla T, Rizzuto R: Chaperone-mediated coupling of endoplasmic reticulum and mitochondrial Ca2+ channels. J Cell Biol. 2006, 175 (6): 901-11. 10.1083/jcb.200608073.PubMed CentralView ArticlePubMedGoogle Scholar
- Hayashi T, Su TP: Sigma-1 receptor chaperones at the ER-mitochondrion interface regulate Ca2+ signaling and cell survival. Cell. 2007, 131 (3): 596-610. 10.1016/j.cell.2007.08.036.View ArticlePubMedGoogle Scholar
- Marchi S, Rimessi A, Giorgi C, Baldini C, Ferroni L, Rizzuto R, Pinton P: Akt kinase reducing endoplasmic reticulum Ca2+ release protects cells from Ca2+-dependent apoptotic stimuli. Biochem Biophys Res Commun. 2008, 375 (4): 501-5. 10.1016/j.bbrc.2008.07.153.PubMed CentralView ArticlePubMedGoogle Scholar
- Boehning D, Patterson RL, Sedaghat L, Glebova NO, Kurosaki T, Snyder SH: Cytochrome c binds to inositol (1,4,5) trisphosphate receptors, amplifying calcium-dependent apoptosis. Nat Cell Biol. 2003, 5 (12): 1051-61. 10.1038/ncb1063.View ArticlePubMedGoogle Scholar
- Giorgio M, Migliaccio E, Orsini F, Paolucci D, Moroni M, Contursi C, Pelliccia G, Luzi L, Minucci S, Marcaccio M, Pinton P, Rizzuto R, Bernardi P, Paolucci F, Pelicci PG: Electron transfer between cytochrome c and p66Shc generates reactive oxygen species that trigger mitochondrial apoptosis. Cell. 2005, 122 (2): 221-33. 10.1016/j.cell.2005.05.011.View ArticlePubMedGoogle Scholar
- Pinton P, Rimessi A, Marchi S, Orsini F, Migliaccio E, Giorgio M, Contursi C, Minucci S, Mantovani F, Wieckowski MR, Del Sal G, Pelicci PG, Rizzuto R: Protein kinase C beta and prolyl isomerase 1 regulate mitochondrial effects of the life-span determinant p66Shc. Science. 2007, 315 (5812): 659-63. 10.1126/science.1135380.View ArticlePubMedGoogle Scholar
- Lebiedzinska M, Karkucinska-Wieckowska A, Giorgi C, Karczmarewicz E, Pronicka E, Pinton P, Duszynski J, Pronicki M, Wieckowski MR: Oxidative stress-dependent p66Shc phosphorylation in skin fibroblasts of children with mitochondrial disorders. Biochim Biophys Acta. 2010, 1797 (6-7): 952-60. 10.1016/j.bbabio.2010.03.005.View ArticlePubMedGoogle Scholar
- Lebiedzinska M, Duszynski J, Rizzuto R, Pinton P, Wieckowski MR: Age-related changes in levels of p66Shc and serine 36-phosphorylated p66Shc in organs and mouse tissues. Arch Biochem Biophys. 2009, 486 (1): 73-80. 10.1016/j.abb.2009.03.007.View ArticlePubMedGoogle Scholar
- Giorgi C, Ito K, Lin HK, Santangelo C, Wieckowski MR, Lebiedzinska M, Bononi A, Bonora M, Duszynski J, Bernardi R, Rizzuto R, Tacchetti C, Pinton P, Pandolfi PP: PML regulates apoptosis at endoplasmic reticulum by modulating calcium release. Science. 2010, 330 (6008): 1247-51. 10.1126/science.1189157.PubMed CentralView ArticlePubMedGoogle Scholar
- Wieckowski MR, Szabadkai G, Wasilewski M, Pinton P, Duszyński J, Rizzuto R: Overexpression of adenine nucleotide translocase reduces Ca2+ signal transmission between the ER and mitochondria. Biochem Biophys Res Commun. 2006, 348 (2): 393-9. 10.1016/j.bbrc.2006.07.072.View ArticlePubMedGoogle Scholar
- Breckenridge DG, Stojanovic M, Marcellus RC, Shore GC: Caspase cleavage product of BAP31 induces mitochondrial fission through endoplasmic reticulum calcium signals, enhancing cytochrome c release to the cytosol. J Cell Biol. 2003, 160 (7): 1115-27. 10.1083/jcb.200212059.PubMed CentralView ArticlePubMedGoogle Scholar
- John LM, Lechleiter JD, Camacho P: Differential modulation of SERCA2 isoforms by calreticulin. J Cell Biol. 1998, 142 (4): 963-73. 10.1083/jcb.142.4.963.PubMed CentralView ArticlePubMedGoogle Scholar
- Higo T, Hattori M, Nakamura T, Natsume T, Michikawa T, Mikoshiba K: Subtype-specific and ER lumenal environment-dependent regulation of inositol 1,4,5-trisphosphate receptor type 1 by ERp44. Cell. 2005, 120 (1): 85-98. 10.1016/j.cell.2004.11.048.View ArticlePubMedGoogle Scholar
- Gilady SY, Bui M, Lynes EM, Benson MD, Watts R, Vance JE, Simmen T: Ero1alpha requires oxidizing and normoxic conditions to localize to the mitochondria-associated membrane (MAM). Cell Stress Chaperones. 2010, 15 (5): 619-29. 10.1007/s12192-010-0174-1.PubMed CentralView ArticlePubMedGoogle Scholar
- Rapizzi E, Pinton P, Szabadkai G, Wieckowski MR, Vandecasteele G, Baird G, Tuft RA, Fogarty KE, Rizzuto R: Recombinant expression of the voltage-dependent anion channel enhances the transfer of Ca2+ microdomains to mitochondria. J Cell Biol. 2002, 159 (4): 613-24. 10.1083/jcb.200205091.PubMed CentralView ArticlePubMedGoogle Scholar
- Simmen T, Aslan JE, Blagoveshchenskaya AD, Thomas L, Wan L, Xiang Y, Feliciangeli SF, Hung CH, Crump CM, Thomas G: PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis. EMBO J. 2005, 24 (4): 717-29. 10.1038/sj.emboj.7600559.PubMed CentralView ArticlePubMedGoogle Scholar
- Cui Z, Vance JE, Chen MH, Voelker DR, Vance DE: Cloning and expression of a novel phosphatidylethanolamine N-methyltransferase. A specific biochemical and cytological marker for a unique membrane fraction in rat liver. J Biol Chem. 1993, 268 (22): 16655-63.PubMedGoogle Scholar
- Rusiñol AE, Cui Z, Chen MH, Vance JE: A unique mitochondria-associated membrane fraction from rat liver has a high capacity for lipid synthesis and contains pre-Golgi secretory proteins including nascent lipoproteins. J Biol Chem. 1994, 269 (44): 27494-502.PubMedGoogle Scholar
- Roderick HL, Lechleiter JD, Camacho P: Cytosolic phosphorylation of calnexin controls intracellular Ca2+ oscillations via an interaction with SERCA2b. J Cell Biol. 2000, 149 (6): 1235-48. 10.1083/jcb.149.6.1235.PubMed CentralView ArticlePubMedGoogle Scholar
- Chami M, Oulès B, Szabadkai G, Tacine R, Rizzuto R, Paterlini-Bréchot P: Role of SERCA1 truncated isoform in the proapoptotic calcium transfer from ER to mitochondria during ER stress. Mol Cell. 2008, 32 (5): 641-51. 10.1016/j.molcel.2008.11.014.PubMed CentralView ArticlePubMedGoogle Scholar
- Ardail D, Popa I, Bodennec J, Louisot P, Schmitt D, Portoukalian J: The mitochondria-associated endoplasmic-reticulum subcompartment (MAM fraction) of rat liver contains highly active sphingolipid-specific glycosyltransferases. Biochem J. 2003, 371 (Pt 3): 1013-9.PubMed CentralView ArticlePubMedGoogle Scholar
- Kuge O, Yamakawa Y, Nishijima M: Enhancement of transport-dependent decarboxylation of phosphatidylserine by S100B protein in permeabilized Chinese hamster ovary cells. J Biol Chem. 2001, 276 (26): 23700-6. 10.1074/jbc.M101911200.View ArticlePubMedGoogle Scholar
- Voelker DR: Bridging gaps in phospholipid transport. Trends Biochem Sci. 2005, 30 (7): 396-404. 10.1016/j.tibs.2005.05.008.View ArticlePubMedGoogle Scholar
- Schmitt M, Grand-Perret T: Regulated turnover of a cell surface-associated pool of newly synthesized apolipoprotein E in HepG2 cells. J Lipid Res. 1999, 40 (1): 39-49.PubMedGoogle Scholar
- Hayashi T, Su TP: Regulating ankyrin dynamics: Roles of sigma-1 receptors. Proc Natl Acad Sci USA. 2001, 98 (2): 491-6. 10.1073/pnas.021413698.PubMed CentralView ArticlePubMedGoogle Scholar
- Myhill N, Lynes EM, Nanji JA, Blagoveshchenskaya AD, Fei H, Carmine-Simmen K, Cooper TJ, Thomas G, Simmen T: The subcellular distribution of calnexin is mediated by PACS-2. Mol Biol Cell. 2008, 19 (7): 2777-88. 10.1091/mbc.E07-10-0995.PubMed CentralView ArticlePubMedGoogle Scholar
- Pinton P, Rizzuto R: Bcl-2 and Ca2+ homeostasis in the endoplasmic reticulum. Cell Death Differ. 2006, 13 (8): 1409-18. 10.1038/sj.cdd.4401960.View ArticlePubMedGoogle Scholar
- Ardail D, Gasnier F, Lermé F, Simonot C, Louisot P, Gateau-Roesch O: Involvement of mitochondrial contact sites in the subcellular compartmentalization of phospholipid biosynthetic enzymes. J Biol Chem. 1993, 268 (34): 25985-92.PubMedGoogle Scholar
- Eilers M, Endo T, Schatz G: Adriamycin, a drug interacting with acidic phospholipids, blocks import of precursor proteins by isolated yeast mitochondria. J Biol Chem. 1989, 264 (5): 2945-50.PubMedGoogle Scholar
- Bozidis P, Williamson CD, Colberg-Poley AM: Mitochondrial and secretory human cytomegalovirus UL37 proteins traffic into mitochondrion-associated membranes of human cells. J Virol. 2008, 82 (6): 2715-26. 10.1128/JVI.02456-07.PubMed CentralView ArticlePubMedGoogle Scholar
- Sheikh MY, Choi J, Qadri I, Friedman JE, Sanyal AJ: Hepatitis C virus infection: molecular pathways to metabolic syndrome. Hepatology. 2008, 47 (6): 2127-33. 10.1002/hep.22269.View ArticlePubMedGoogle Scholar
- Li G, Mongillo M, Chin KT, Harding H, Ron D, Marks AR, Tabas I: Role of ERO1-alpha-mediated stimulation of inositol 1,4,5-triphosphate receptor activity in endoplasmic reticulum stress-induced apoptosis. J Cell Biol. 2009, 186 (6): 783-92. 10.1083/jcb.200904060.PubMed CentralView ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.